NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Polycomb mutant seedlings
Description:We have identified a histone methyltransferase gene CURLY LEAF (CLF), which is required to repress a floral homeotic gene AGAMOUS (AG). CLF acts redundantly with a related gene SWINGER (SWN), so that clf swn double mutants have extreme, near lethal phenotypes in which expression of many target genes is disrupted. To understand better when and where these genes act, we propose to use a system for generating mosaic plants, in which marked sectors of cells are created that gain or lose CLF (or other Pc-G) gene function in SWN+ and swn- mutant backgrounds. This will allow us to address questions such as whether the Pc-G genes are needed throughout development to silence their targets, whether they are needed once cell division has ceased, whether they can silence their targets once the targets have been activated, and whether genes that are epigenetically silenced by CLF are impervious to activating factors.Source: Justin Goodrich staff homepage Aim is to compare gene expression in wild type and polycomb mutant seedlings. Comparison will be of bulked seedlings of four genotypes: Columbia, clf-28, swn-7, clf-28 swn-7. Tissue will be grown in tissue culture.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Goodrich_1-1_Col_Rep1_ATH121055whole plant N1092Goodrich_1-1_Col_Rep1_ATH1.CEL
Goodrich_1-2_curly-leaf_Rep1_ATH121056T-DNA insertion mutantwhole plant SALK_050195Goodrich_1-2_curly-leaf_Rep1_ATH1.CEL
Goodrich_1-3_swinger_Rep1_ATH121057T-DNA insertion mutantwhole plant SALK_021003Goodrich_1-3_swinger_Rep1_ATH1.CEL
Goodrich_1-4_curly-leaf-swinger_Rep1_ATH121058T-DNA insertion mutantwhole plant Goodrich_1-4_curly-leaf-swinger_Rep1_ATH1.CEL
Goodrich_1-5_Col_Rep2_ATH121059whole plant N1092Goodrich_1-5_Col_Rep2_ATH1.CEL
Goodrich_1-6_curly-leaf_Rep2_ATH121060T-DNA insertion mutantwhole plant SALK_021003Goodrich_1-6_curly-leaf_Rep2_ATH1.CEL
Goodrich_1-7_swinger_Rep2_ATH121061T-DNA insertion mutantwhole plant SALK_050195Goodrich_1-7_swinger_Rep2_ATH1.CEL
Goodrich_1-8_curly-leaf-swinger_Rep2_ATH121062T-DNA insertion mutantwhole plant Goodrich_1-8_curly-leaf-swinger_Rep2_ATH1.CEL