| Description: | Previous microarray analyses had revealed that myriad of genes are regulated under the circadian clock. These transcripts appear peak abundances at all phases of the subjective day and night, indicating that a complex network of transcriptional factors is used to regulate these transcripts throughout the various phases. The clock core to generate circadian rhythmic gene expression relies on the interaction of genetic feedback loops. The PSEUDO RESPONSE REGULATOR (PRR) gene family including TIMING OF CAB EXPRESSION 1 (TOC1) is involved in the mechanism. Indeed, prr9-10 prr7-11 prr5-11 (d975) triple mutant showed the arrhythmicity of circadian-associated genes expression. Prr9-10 is the SALK_007557 T-DNA insertion line, whose insertion is located the second exon of PRR9 coding sequence. Prr7-11 is the SALK_030430 T-DNA insertion line, whose insertion is located the first exon of PRR7 coding sequence. Prr5-11 is the KAZUSA_KG24599 T-DNA insertion line, whose insertion is located the fourth exon of PRR5 coding sequence. In this study, we use microarray analyses employing the d975, prr9-10 prr7-11 (d97), prr9-10 prr5-11 (d95), prr7-11 prr5-11 (d75), 35S::PRR5 (5ox), 35S::PRR1 (=35S::TOC1) (1ox), phyB-9 (phyB), and wild type (Col-0) to describe the global CCG regulatory network. These plants were grown on MS plate containing 2% sucrose for 18 days under constant light conditions. Under the conditions, the circadian phases of the plants in the same sample were different from each other because the germination timing differs between plants. Therefore, the gene expression level of CCGs should be a diurnal average (or mixed) level. Three independent biological samples of plants were harvested in 8h intervals. The RNA samples were prepared from the plants with use of RNeasy Plant Mini Kit (Qiagen, Valencia, CA). The quality of RNA prepared was analyzed by Bioanalyzer 2100 (Agilent Technologies). These RNA samples were processed as recommended by the Affymetrix instruction (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetrix). These dataset will provide us with bases for understanding the global CCG regulatory network by PRR9, PRR7, and PRR5. |
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
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