NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Signaling from an Altered Cell Wall to the Nucleus Mediates Sugar-responsive Growth and Development
Description:Sugars such as glucose function as signal molecules that regulate gene expression, growth and development in plants, animals and yeast. To understand the molecular mechanisms of sugar responses, we isolated and characterized an Arabidopsis thaliana mutant, high sugar- response 8 (hsr8), which enhances sugar responsive growth and gene expression. Light- grown hsr8 plants exhibited increased starch and anthocyanin and reduced chlorophyll content in response to glucose treatment. Dark- grown hsr8 seedlings show glucose- hypersensitive hypocotyl elongation and development. The HSR8 gene, isolated using map-based cloning, is allelic to the MUR4 gene involved in arabinose synthesis. Dark- grown mur1 and mur3 seedlings also exhibit similar sugar responses to hsr8/mur4. The sugar- hypersensitive phenotypes of hsr8/mur4, mur1 and mur3 were restored to those of WT by boric acid, suggesting that alterations in the cell wall cause hypersensitive sugar- responsive phenotypes. Genetic analysis showed that sugar- hypersensitive responses in hsr8 mutants were suppressed by prl1, indicating that nuclear-localised PRL1 is required for enhanced sugar-responses in hsr8 mutant plants. Microarray analysis revealed that expression of many cell wall-related and sugar-responsive genes was altered in mur4-1, and expression of a significant proportion of these genes was restored to wild type levels in the mur4-1prl1 double mutant. These findings reveal a pathway that signals changes in the cell wall through PRL1 to altered gene expression and sugar- responsive metabolic, growth and developmental changes. The above experiments are conducted on 6d-old seedlings grown on medium containning 1% glucose in dark.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Li_1-1_col_Rep1_ATH120934whole plant Li_1-1_col_Rep1_ATH1.CEL
Li_1-2_col_Rep2_ATH120935whole plant Li_1-2_col_Rep2_ATH1.CEL
Li_1-3_mur4-1_Rep1_ATH120936Col-0whole plant N8568Li_1-3_mur4-1_Rep1_ATH1.CEL
Li_1-4_mur4-1_Rep2_ATH120937Col-0whole plant N8568Li_1-4_mur4-1_Rep2_ATH1.CEL
Li_1-5_prl1_Rep1_ATH120938Col-0whole plant SALK_039427Li_1-5_prl1_Rep1_ATH1.CEL
Li_1-6_prl1_Rep2_ATH120939Col-0whole plant SALK_039427Li_1-6_prl1_Rep2_ATH1.CEL
Li_1-7_mur4-1prl1_Rep1_ATH120940Col-0whole plant Li_1-7_mur4-1prl1_Rep1_ATH1.CEL
Li_1-8_mur4-1prl1_Rep2_ATH120941Col-0whole plant Li_1-8_mur4-1prl1_Rep2_ATH1.CEL