NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	417
Title:	Microarray analysis of Arabidopsis plants inoculated with HopAO1 mutants of Pst DC3000
Date:	2007-02-28
Description:	This experiment was performed to identify gene expression changes in wild type (Col-0) Arabidopsis plants after inoculation with Pseudomonas syringae pv. tomato (Pst) strain DC3000 or a mutant derivative lacking the type III effector tyrosine phosphatase HopAO1. Col-0 Arabidopsis plants were inoculated by vacuum-infiltration with suspensions of Pst DC3000 or the deltaHopAO1 mutant at 10e8 cfu/ml or with a mock inoculum of sterile water. Inoculated leaf tissues were harvested 7 hours after infiltration and used for RNA isolation and subsequent microarray analysis using Affymetrix ATH1 genechips.
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Tissue	Stock Code	Cel File
Underwood_3-1_Col0-PstDC3000-inocu_Rep1_ATH1	20919	Leaves	N1092	Underwood_3-1_Col0-PstDC3000-inocu_Rep1_ATH1.CEL
Underwood_3-1_Col0-PstDC3000-inocu_Rep1_ATH1	Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of Pst DC3000 in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_3-2_Col0-PstDC3000-inocu_Rep2_ATH1	20920	Leaves	N1092	Underwood_3-2_Col0-PstDC3000-inocu_Rep2_ATH1.CEL
Underwood_3-2_Col0-PstDC3000-inocu_Rep2_ATH1	Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of Pst DC3000 in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_3-3_Col0-PstDC3000-inocu_Rep3_ATH1	20921	Leaves	N1092	Underwood_3-3_Col0-PstDC3000-inocu_Rep3_ATH1.CEL
Underwood_3-3_Col0-PstDC3000-inocu_Rep3_ATH1	Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of Pst DC3000 in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_3-4_Col0-PstDC3000-deltaHopAO1-inocu_Rep1_ATH1	20922	Leaves	N1092	Underwood_3-4_Col0-PstDC3000-deltaHopAO1-inocu_Rep1_ATH1.CEL
Underwood_3-4_Col0-PstDC3000-deltaHopAO1-inocu_Rep1_ATH1	Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 deltaHopAO1 mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_3-5_Col0-PstDC3000-deltaHopAO1-inocu_Rep2_ATH1	20923	Leaves	N1092	Underwood_3-5_Col0-PstDC3000-deltaHopAO1-inocu_Rep2_ATH1.CEL
Underwood_3-5_Col0-PstDC3000-deltaHopAO1-inocu_Rep2_ATH1	Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 deltaHopAO1 mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_3-6_Col0-PstDC3000-deltaHopAO1-inocu_Rep3_ATH1	20924	Leaves	N1092	Underwood_3-6_Col0-PstDC3000-deltaHopAO1-inocu_Rep3_ATH1.CEL
Underwood_3-6_Col0-PstDC3000-deltaHopAO1-inocu_Rep3_ATH1	Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 deltaHopAO1 mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_3-7_Col0-H2O-mock-inocu_Rep1_ATH1	20925	Leaves	N1092	Underwood_3-7_Col0-H2O-mock-inocu_Rep1_ATH1.CEL
Underwood_3-7_Col0-H2O-mock-inocu_Rep1_ATH1	Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_3-8_Col0-H2O-mock-inocu_Rep2_ATH1	20926	Leaves	N1092	Underwood_3-8_Col0-H2O-mock-inocu_Rep2_ATH1.CEL
Underwood_3-8_Col0-H2O-mock-inocu_Rep2_ATH1	Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_3-9_Col0-H2O-mock-inocu_Rep3_ATH1	20927	Leaves	N1092	Underwood_3-9_Col0-H2O-mock-inocu_Rep3_ATH1.CEL
Underwood_3-9_Col0-H2O-mock-inocu_Rep3_ATH1	Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.