NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:416
Title:Microarray analysis of HopAO1 transgenic Arabidopsis plants
Date:2007-02-28
Description:This experiment was performed to identify gene expression changes resulting from the expression of the Pseudomonas syringae pv. tomato (Pst) strain DC3000 type III effector tyrosine phosphatase HopAO1 in stable Arabidopsis transgenic lines. Wild-type (Col gl1) plants and transgenic plants expressing HopAO1 or a mutant derivative, HopAO1 C378S, lacking phosphatase catalytic activity were vacuum-infiltrated with either a mock inoculum of sterile water or a suspension of the Pst DC3000 hrpA mutant and changes in gene expression in infiltrated leaf tissues were investigated 7 hours after inoculation using Affymetrix ATH1 genechips.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Underwood_2-10_Col-gl1-hrpA-inocu_Rep1_ATH120910Leaves N1644Underwood_2-10_Col-gl1-hrpA-inocu_Rep1_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 hrpA mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_2-11_Col-gl1-hrpA-inocu_Rep2_ATH120911Leaves N1644Underwood_2-11_Col-gl1-hrpA-inocu_Rep2_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 hrpA mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_2-12_Col-gl1-hrpA-inocu_Rep3_ATH120912Leaves N1644Underwood_2-12_Col-gl1-hrpA-inocu_Rep3_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 hrpA mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_2-13_HopAO1-hrpA-inocu_Rep1_ATH120913Col-5Leaves Underwood_2-13_HopAO1-hrpA-inocu_Rep1_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 hrpA mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_2-14_HopAO1-hrpA-inocu_Rep2_ATH120914Col-5Leaves Underwood_2-14_HopAO1-hrpA-inocu_Rep2_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 hrpA mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_2-15_HopAO1-hrpA-inocu_Rep3_ATH120915Col-5Leaves Underwood_2-15_HopAO1-hrpA-inocu_Rep3_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 hrpA mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation.
Underwood_2-16_HopAO1-C378S-hrpA-inocu_Rep1_ATH120916Col-5Leaves Underwood_2-16_HopAO1-C378S-hrpA-inocu_Rep1_ATH1.CEL
Treatment: Total RNA was extracted from 1g of frozen leaf tissue using the Promega RNAgents kit according to the manufacturers protocol. Isolated RNA was further purified prior to labeling using the Qiagen RNeasy spin column kit according to the manufacturers protocol.
Underwood_2-17_HopAO1-C378S-hrpA-inocu_Rep2_ATH120917Col-5Leaves Underwood_2-17_HopAO1-C378S-hrpA-inocu_Rep2_ATH1.CEL
Treatment: Total RNA was extracted from 1g of frozen leaf tissue using the Promega RNAgents kit according to the manufacturers protocol. Isolated RNA was further purified prior to labeling using the Qiagen RNeasy spin column kit according to the manufacturers protocol.
Underwood_2-18_HopAO1-C378S-hrpA-inocu_Rep3_ATH120918Col-5Leaves Underwood_2-18_HopAO1-C378S-hrpA-inocu_Rep3_ATH1.CEL
Treatment: Total RNA was extracted from 1g of frozen leaf tissue using the Promega RNAgents kit according to the manufacturers protocol. Isolated RNA was further purified prior to labeling using the Qiagen RNeasy spin column kit according to the manufacturers protocol.
Underwood_2-1_Col-gl1-mock-inocu_Rep1_ATH120901Leaves N1644Underwood_2-1_Col-gl1-mock-inocu_Rep1_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing the surfactant Silwet L-77 at a concentration of .004%. Inoculated leaf tissue from at least 15 plants was collected for RNA isolation.
Underwood_2-2_Col-gl1-mock-inocu_Rep2_ATH120902Leaves N1644Underwood_2-2_Col-gl1-mock-inocu_Rep2_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing the surfactant Silwet L-77 at a concentration of .004%. Inoculated leaf tissue from at least 15 plants was collected for RNA isolation.
Underwood_2-3_Col-gl1-mock-inocu_Rep3_ATH120903Leaves N1644Underwood_2-3_Col-gl1-mock-inocu_Rep3_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing the surfactant Silwet L-77 at a concentration of .004%. Inoculated leaf tissue from at least 15 plants was collected for RNA isolation.
Underwood_2-4_HopAO1-mock-inocu_Rep1_ATH120904Col-5Leaves Underwood_2-4_HopAO1-mock-inocu_Rep1_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing the surfactant Silwet L-77 at a concentration of .004%. Inoculated leaf tissue from at least 15 plants was collected for RNA isolation
Underwood_2-5_HopAO1-mock-inocu_Rep2_ATH120905Col-5Leaves Underwood_2-5_HopAO1-mock-inocu_Rep2_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing the surfactant Silwet L-77 at a concentration of .004%. Inoculated leaf tissue from at least 15 plants was collected for RNA isolation
Underwood_2-6_HopAO1-mock-inocu_Rep3_ATH120906Col-5Leaves Underwood_2-6_HopAO1-mock-inocu_Rep3_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing the surfactant Silwet L-77 at a concentration of .004%. Inoculated leaf tissue from at least 15 plants was collected for RNA isolation
Underwood_2-7_HopAO1-C378S-mock-inocu_Rep1_ATH120907Col-5Leaves Underwood_2-7_HopAO1-C378S-mock-inocu_Rep1_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing the surfactant Silwet L-77 at a concentration of .004%. Inoculated leaf tissue from at least 15 plants was collected for RNA isolation.
Underwood_2-8_HopAO1-C378S-mock-inocu_Rep2_ATH120908Col-5Leaves Underwood_2-8_HopAO1-C378S-mock-inocu_Rep2_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing the surfactant Silwet L-77 at a concentration of .004%. Inoculated leaf tissue from at least 15 plants was collected for RNA isolation.
Underwood_2-9_HopAO1-C378S-mock-inocu_Rep3_ATH120909Col-5Leaves Underwood_2-9_HopAO1-C378S-mock-inocu_Rep3_ATH1.CEL
Treatment: Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing the surfactant Silwet L-77 at a concentration of .004%. Inoculated leaf tissue from at least 15 plants was collected for RNA isolation.