NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:415
Title:Effects of the cucumber mosaic virus 2b silencing suppressor protein on inducible plant defence path
Date:2008-07-30
Description:In this study, we have examined the effects of the cucumber mosaic virus 2b protein on the salicylic acid (SA) and methyl jasmonate (meJa) induced defence pathways in A. thaliana. We have also investigated the effects of the 2b protein on antimycin A-induced defence, which represents a virus-specific branch of SA-induced defence. Transgenic A. thaliana (ecotype Col 0) expressing the 2b gene from CMV (strain Fny) were treated independently with SA, meJa and AA, as were wild-type controls. After 25 hours, RNA was extracted from aerial tissue for comparison of gene expression profiles in the presence and absence of the 2b protein. The 2b protein is known to suppress SA-induced resistance to plant viruses as well as the siRNA and miRNA-mediated gene silencing pathways. It is able to suppress SA-mediated induction of alternative oxidase, a gene that functions in the AA-induced virus-specific branch of SA-induced defence (Ji and Ding, 2001, Mol. Plant Microbe Interactions 14, 715-724). Furthermore, the 2b protein is the major determinant of viral symptoms in CMV and causes symptom-like alterations in the phenotype of Arabidopsis thaliana plants expressing 2b transgenes (Lewsey et al., 2007, Plant J., in press). However, its effects on the meJa-induced defence pathway, which has crosstalk with the SA-induced defence pathway, are not known.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Lewsey_1-10_Fny-CMV2b-H2O_Rep2_ATH120886Col-0all aerial tissues Lewsey_1-10_Fny-CMV2b-H2O_Rep2_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-11_Wild-type-salicylic-acid_Rep2_ATH120887all aerial tissues Lewsey_1-11_Wild-type-salicylic-acid_Rep2_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 1mM salicylic acid and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction
Lewsey_1-12_Fny-CMV2b-salicylic-acid_Rep2_ATH120888Col-0all aerial tissues Lewsey_1-12_Fny-CMV2b-salicylic-acid_Rep2_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 1mM salicylic acid and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-13_Wild-type-antimycinA_Rep2_ATH120889all aerial tissues Lewsey_1-13_Wild-type-antimycinA_Rep2_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 50 micromolar antimycin A and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-14_Fny-CMV2b-antimycin-A_Rep2_ATH120890Col-0all aerial tissues Lewsey_1-14_Fny-CMV2b-antimycin-A_Rep2_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 50 micromolar antimycin A and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-15_Wild-type-methyl-jasmonate_Rep2_ATH120891all aerial tissues Lewsey_1-15_Wild-type-methyl-jasmonate_Rep2_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 250 micromolar methyl jasmonate and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-16_Fny-CMV2b-methyl-jasmonate_Rep2_ATH120892Col-0all aerial tissues Lewsey_1-16_Fny-CMV2b-methyl-jasmonate_Rep2_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 250 micromolar methyl jasmonate and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-17_Wild-type-H2O_Rep3_ATH120893all aerial tissues Lewsey_1-17_Wild-type-H2O_Rep3_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-18_Fny-CMV2b-H2O_Rep3_ATH120894Col-0all aerial tissues Lewsey_1-18_Fny-CMV2b-H2O_Rep3_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-19_Wild-type-salicylic-acid_Rep3_ATH120895all aerial tissues Lewsey_1-19_Wild-type-salicylic-acid_Rep3_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 1mM salicylic acid and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction
Lewsey_1-1_Wild-type-H2O_Rep1_ATH120877all aerial tissues Lewsey_1-1_Wild-type-H2O_Rep1_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-20_Fny-CMV2b-salicylic-acid_Rep3_ATH120896Col-0all aerial tissues Lewsey_1-20_Fny-CMV2b-salicylic-acid_Rep3_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 1mM salicylic acid and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-21_Wild-type-antimycinA_Rep3_ATH120897all aerial tissues Lewsey_1-21_Wild-type-antimycinA_Rep3_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 50 micromolar antimycin A and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-22_Fny-CMV2b-antimycin-A_Rep3_ATH120898Col-0all aerial tissues Lewsey_1-22_Fny-CMV2b-antimycin-A_Rep3_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 50 micromolar antimycin A and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-23_Wild-type-methyl-jasmonate_Rep3_ATH120899all aerial tissues Lewsey_1-23_Wild-type-methyl-jasmonate_Rep3_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 250 micromolar methyl jasmonate and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-24_Fny-CMV2b-methyl-jasmonate_Rep3_ATH120900Col-0all aerial tissues Lewsey_1-24_Fny-CMV2b-methyl-jasmonate_Rep3_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 250 micromolar methyl jasmonate and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-2_Fny-CMV2b-H2O_Rep1_ATH120878Col-0all aerial tissues Lewsey_1-2_Fny-CMV2b-H2O_Rep1_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-3_Wild-type-salicylic-acid_Rep1_ATH120879all aerial tissues Lewsey_1-3_Wild-type-salicylic-acid_Rep1_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 1mM salicylic acid and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction
Lewsey_1-4_Fny-CMV2b-salicylic-acid_Rep1_ATH120880Col-0all aerial tissues Lewsey_1-4_Fny-CMV2b-salicylic-acid_Rep1_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 1mM salicylic acid and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-5_Wild-type-antimycinA_Rep1_ATH120881all aerial tissues Lewsey_1-5_Wild-type-antimycinA_Rep1_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 50 micromolar antimycin A and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-6_Fny-CMV2b-antimycin-A_Rep1_ATH120882Col-0all aerial tissues Lewsey_1-6_Fny-CMV2b-antimycin-A_Rep1_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 50 micromolar antimycin A and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-7_Wild-type-methyl-jasmonate_Rep1_ATH120883all aerial tissues Lewsey_1-7_Wild-type-methyl-jasmonate_Rep1_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 250 micromolar methyl jasmonate and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-8_Fny-CMV2b-methyl-jasmonate_Rep1_ATH120884Col-0all aerial tissues Lewsey_1-8_Fny-CMV2b-methyl-jasmonate_Rep1_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 250 micromolar methyl jasmonate and 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.
Lewsey_1-9_Wild-type-H2O_Rep2_ATH120885all aerial tissues Lewsey_1-9_Wild-type-H2O_Rep2_ATH1.CEL
Treatment: Approximately ten plants were sprayed with water containing 0.1% (v/v) ethanol, 5.5 hrs into the light cycle, when plants were 6 weeks old (growth stage 3.50 to 3.70). Plant tissues were harvested 25 hours after treatment and pooled to minimise variation. The pooled tissue was then used for RNA extraction.