NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	413
Title:	Cis-jasmone as a novel signalling molecule in plant gene expression
Date:	2008-07-28
Description:	(Z)-Jasmone (cis-jasmone) is a highly volatile product of jasmonic acid (JA) catabolism and is not only a constitutive compound of flower volatiles but also induced by insect damage of vegetative tissue. It has been speculated that cis-jasmone might be a biologically inactive sink for the JA pathway but recently it was demonstrated that cis-jasmone acts as an insect semiochemical. In addition, we have shown by differential display that cis-jasmone induces plant gene expression qualitatively distinct from that elicited by methyl jasmonic acid (Birkett et al, PNAS 97: 9329-9334). Thus it is likely that cis-jasmone is a new lipid-derived signalling compound as opposed to the previously proposed inert sink.We are therefore interested in using the Arabidopsis DNA microarray to search for genes whose expression is altered by cis-jasmone treatment. As it is not known yet what role cis-jasmone might play in oxylipin signalling, we hope that the microarray identification of such genes will give an indication as to whether cis-jasmone is involved solely in defence signalling or whether it has additional functions.Arabidopsis thaliana ecotype Columbia will be exposed to cis-jasmone for 1 or 3 hours respectively. For each treatment (and the mock treated control plants) 8 rosettes will be harvested, pooled and used for RNA extraction. Three biological replicates will be generated per treatment.
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Tissue	Stock Code	Cel File
Matthes_1-10_cis-Jasmone-3hr_Rep3_ATH1	20848	whole plant	N1092	Matthes_1-10_cis-Jasmone-3hr_Rep3_ATH1.CEL
Matthes_1-10_cis-Jasmone-3hr_Rep3_ATH1	Treatment: The plants were put into an air tight container. On the lid of the container a small piece of 3 mm Whatman paper was attached and 1.5 ul of cis-jasmone were applied. The lid was put back onto the container and sealed.
Matthes_1-1_control-1hr_Rep1_ATH1	20839	whole plant	N1092	Matthes_1-1_control-1hr_Rep1_ATH1.CEL
Matthes_1-1_control-1hr_Rep1_ATH1	Treatment: As this sample is a control for the effect of the volatile cis-jasmone on Arabidopsis, the plants were kept in an air-tight container for the indicated time but without adding the compound.
Matthes_1-2_control-1hr_Rep2_ATH1	20840	whole plant	N1092	Matthes_1-2_control-1hr_Rep2_ATH1.CEL
Matthes_1-2_control-1hr_Rep2_ATH1	Treatment: As this sample is a control for the effect of the volatile cis-jasmone on Arabidopsis, the plants were kept in an air-tight container for the indicated time but without adding the compound.
Matthes_1-3_cis-Jasmone-1hr_Rep1_ATH1	20841	whole plant	N1092	Matthes_1-3_cis-Jasmone-1hr_Rep1_ATH1.CEL
Matthes_1-3_cis-Jasmone-1hr_Rep1_ATH1	Treatment: The plants were put into an air tight container. On the lid of the container a small piece of 3 mm Whatman paper was attached and 1.5 ul of cis-jasmone were applied. The lid was put back onto the container and sealed.
Matthes_1-4_cis-Jasmone-1hr_Rep2_ATH1	20842	whole plant	N1092	Matthes_1-4_cis-Jasmone-1hr_Rep2_ATH1.CEL
Matthes_1-4_cis-Jasmone-1hr_Rep2_ATH1	Treatment: The plants were put into an air tight container. On the lid of the container a small piece of 3 mm Whatman paper was attached and 1.5 ul of cis-jasmone were applied. The lid was put back onto the container and sealed.
Matthes_1-5_cis-Jasmone-1hr_Rep3_ATH1	20843	whole plant	N1092	Matthes_1-5_cis-Jasmone-1hr_Rep3_ATH1.CEL
Matthes_1-5_cis-Jasmone-1hr_Rep3_ATH1	Treatment: The plants were put into an air tight container. On the lid of the container a small piece of 3 mm Whatman paper was attached and 1.5 ul of cis-jasmone were applied. The lid was put back onto the container and sealed.
Matthes_1-6_control-3hrs_Rep1_ATH1	20844	whole plant	N1092	Matthes_1-6_control-3hrs_Rep1_ATH1.CEL
Matthes_1-6_control-3hrs_Rep1_ATH1	Treatment: The plants were put into an air tight container. On the lid of the container a small piece of 3 mm Whatman paper was attached and 1.5 ul of cis-jasmone were applied. The lid was put back onto the container and sealed.
Matthes_1-7_control-3hrs_Rep2_ATH1	20845	whole plant	N1092	Matthes_1-7_control-3hrs_Rep2_ATH1.CEL
Matthes_1-7_control-3hrs_Rep2_ATH1	Treatment: The plants were put into an air tight container. On the lid of the container a small piece of 3 mm Whatman paper was attached and 1.5 ul of cis-jasmone were applied. The lid was put back onto the container and sealed.
Matthes_1-8_cis-Jasmone-3hr_Rep1_ATH1	20846	whole plant	N1092	Matthes_1-8_cis-Jasmone-3hr_Rep1_ATH1.CEL
Matthes_1-8_cis-Jasmone-3hr_Rep1_ATH1	Treatment: The plants were put into an air tight container. On the lid of the container a small piece of 3 mm Whatman paper was attached and 1.5 ul of cis-jasmone were applied. The lid was put back onto the container and sealed.
Matthes_1-9_cis-Jasmone-3hr_Rep2_ATH1	20847	whole plant	N1092	Matthes_1-9_cis-Jasmone-3hr_Rep2_ATH1.CEL
Matthes_1-9_cis-Jasmone-3hr_Rep2_ATH1	Treatment: The plants were put into an air tight container. On the lid of the container a small piece of 3 mm Whatman paper was attached and 1.5 ul of cis-jasmone were applied. The lid was put back onto the container and sealed.