NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Transcript inhibition from limited starch degradation
Description:In starchless mutants of Arabidopsis, pgm, and wild type, Col0, there are small differences in transcript levels at the end of the day, but large differences at the end of the night. Many of the transcripts of genes involved in biosynthesis and growth are repressed in the pgm mutant (Thimm et al., 2004). It is hypothesized that a transient depletion in sugars at the end of the night inhibits carbon utilization at the beginning of the day (Gibon et al., 2004). In order to test if the same inhibition of transcripts involved in biosynthesis and growth also occurs in starch excess mutants of Arabidopsis Affymetrix arrays will be generated for WT (Col0), and the following mutants: sex4-3 (Salk 102567), ptpkis2 (Salk 053285), and sex1-3 (Yu et al., 2001). WT, sex4-3, and ptpkis2 plants were grown for 30 days and sex1-3 plants were grown for 36 days. All plants were grown in a growth chamber with a 12 hour photoperiod, quantum flux 100 mol m-2s-1, relative humidity was at least 75%, and a constant temperature of 20oC. Three leaves from 8 different plants of each genotype were harvested using a green safe lamp 0.5 hours before the lights came on in the morning and immediately frozen in LN2. Leaf material was powered using a mortar and pestle and RNA was extracted using a Purescript RNA isolation kit (Gentra Systems, MN, USA). Samples were then treated with DNaseI (Invitrogen, CA, USA).
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Weise_1-10_Sex1-3_Rep1_ATH120830Col-0Leaves Weise_1-10_Sex1-3_Rep1_ATH1.CEL
Weise_1-11_Sex1-3_Rep2_ATH120831Col-0Leaves Weise_1-11_Sex1-3_Rep2_ATH1.CEL
Weise_1-12_Sex1-3_Rep3_ATH120832Col-0Leaves Weise_1-12_Sex1-3_Rep3_ATH1.CEL
Weise_1-1_Col-WT_Rep1_ATH120821Leaves Weise_1-1_Col-WT_Rep1_ATH1.CEL
Weise_1-2_Col-WT_Rep2_ATH120822Leaves Weise_1-2_Col-WT_Rep2_ATH1.CEL
Weise_1-3_Col-WT_Rep3_ATH120823Leaves Weise_1-3_Col-WT_Rep3_ATH1.CEL
Weise_1-4_ptpks_Rep1_ATH120824Leaves N553285Weise_1-4_ptpks_Rep1_ATH1.CEL
Weise_1-5_ptpks_Rep2_ATH120825Leaves N553285Weise_1-5_ptpks_Rep2_ATH1.CEL
Weise_1-6_ptpks_Rep3_ATH120826Leaves N553285Weise_1-6_ptpks_Rep3_ATH1.CEL
Weise_1-7_Sex4-3_Rep1_ATH120827Leaves N602567Weise_1-7_Sex4-3_Rep1_ATH1.CEL
Weise_1-8_Sex4-3_Rep2_ATH120828Leaves N602567Weise_1-8_Sex4-3_Rep2_ATH1.CEL
Weise_1-9_Sex4-3_Rep3_ATH120829Leaves N602567Weise_1-9_Sex4-3_Rep3_ATH1.CEL