| Experiment: | 406 |
| Title: | Cytokinin treatment on roots of seedlings |
| Date: | 2006-12-06 |
| Description: | According to the well-documented scenario with regard to the cytokinin-mediated phosphorelay signal transduction in Arabidopsis thaliana, certain members of the type-B ARR family are crucially implicated in the regulatory networks that are primarily propagated by the cytokinin-receptors (AHKs) in response to cytokinin. Nevertheless, clarification of the biological impact of these type-B ARR transcription factors is at a very early stage. Here we focused on a pair of highly homologous ARR10 and ARR12 genes by constructing an arr10 and arr12 double null mutant. The mutant alleles used in this study were arr10-5 and arr12-1. arr10-5 is the SALK_098604 T-DNA insertion line, whose mutation was determined to be located in the fifth exon of the ARR10 coding sequence. arr12-1 is the SALK_054752 T-DNA insertion line, whose mutation was determined to be located in the third exon of the ARR12 coding sequence. The resulting mutant showed remarkable phenotypes with special reference to the cytokinin-action in roots (e.g., inhibition of root elongation, green callus formation from explants). Furthermore, we demonstrated that ARR10 and ARR12 are involved in the AHK-dependent signaling pathway that modulates the differentiation of root-vascular tissues (i.e., protoxylem-specification), suggesting that ARR10 and ARR12 are the prominent players that act redundantly in the AHK-dependent cytokinin signaling in roots. Keeping this in mind, we then collected the root-specific and combinatorial DNA microarray datasets with regard to the cytokinin-responsible genes by employing both the wild-type and arr10 arr12 double mutant plants. In this study, wild type and the arr10 arr12 mutant grown vertically on MS agar plates for 2 weeks were treated with 20 microM trans-zeatin or 0.02% DMSO (solvent for trans-zeatin solution) for 1h. These treated plant samples were divided into three portions, from which RNA samples were prepared separately from roots of seedlings with use of RNeasy Plant Mini Kit (Qiagen, Valencia, CA, U.S.A.). The Quality of RNAs prepared was analyzed by Bioanalyzer 2100 (Agilent Technologies). These RNA samples were processed as recommended by the Affymetrix instruction (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetrix). These dataset will provide us with bases for understanding the early response to cytokinin on roots of seedlings in Arabidopsis thaliana. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|
| Sakakibara_2-10_DMSO-treatment-mutant_Rep1_ATH1 | 20788 | | roots | N598604, N554752 | Sakakibara_2-10_DMSO-treatment-mutant_Rep1_ATH1.cel |
| Treatment: The Arabidopsis were treated with 0.02% DMSO by spraying for 1h |
| Sakakibara_2-11_DMSO-treatment-mutant_Rep2_ATH1 | 20789 | | roots | N598604, N554752 | Sakakibara_2-11_DMSO-treatment-mutant_Rep2_ATH1.cel |
| Treatment: The Arabidopsis were treated with 0.02% DMSO by spraying for 1h |
| Sakakibara_2-12_DMSO-treatment-mutant_Rep3_ATH1 | 20790 | | roots | N598604, N554752 | Sakakibara_2-12_DMSO-treatment-mutant_Rep3_ATH1.cel |
| Treatment: The Arabidopsis were treated with 0.02% DMSO by spraying for 1h |
| Sakakibara_2-1_CK-treatment-wildtype_Rep1_ATH1 | 20779 | | roots | N1092 | Sakakibara_2-1_CK-treatment-wildtype_Rep1_ATH1.cel |
| Treatment: The Arabidopsis were treated with 20 µM trans-zeatin by spraying for 1h |
| Sakakibara_2-2_CK-treatment-wildtype_Rep2_ATH1 | 20780 | | roots | N1092 | Sakakibara_2-2_CK-treatment-wildtype_Rep2_ATH1.cel |
| Treatment: The Arabidopsis were treated with 20 µM trans-zeatin by spraying for 1h |
| Sakakibara_2-3_CK-treatment-wildtype_Rep3_ATH1 | 20781 | | roots | N1092 | Sakakibara_2-3_CK-treatment-wildtype_Rep3_ATH1.cel |
| Treatment: The Arabidopsis were treated with 20 µM trans-zeatin by spraying for 1h |
| Sakakibara_2-4_DMSO-treatment-wildtype_Rep1_ATH1 | 20782 | | roots | N1092 | Sakakibara_2-4_DMSO-treatment-wildtype_Rep1_ATH1.cel |
| Treatment: The Arabidopsis were treated with 0.02% DMSO by spraying for 1h |
| Sakakibara_2-5_DMSO-treatment-wildtype_Rep2_ATH1 | 20783 | | roots | N1092 | Sakakibara_2-5_DMSO-treatment-wildtype_Rep2_ATH1.cel |
| Treatment: The Arabidopsis were treated with 0.02% DMSO by spraying for 1h |
| Sakakibara_2-6_DMSO-treatment-wildtype_Rep3_ATH1 | 20784 | | roots | N1092 | Sakakibara_2-6_DMSO-treatment-wildtype_Rep3_ATH1.cel |
| Treatment: The Arabidopsis were treated with 0.02% DMSO by spraying for 1h |
| Sakakibara_2-7_TZ-treatment-mutant_Rep1_ATH1 | 20785 | | roots | N598604, N554752 | Sakakibara_2-7_TZ-treatment-mutant_Rep1_ATH1.cel |
| Treatment: The Arabidopsis were treated with 20 micro M trans-zeatin by spraying for 1h |
| Sakakibara_2-8_TZ-treatment-mutant_Rep2_ATH1 | 20786 | | roots | N598604, N554752 | Sakakibara_2-8_TZ-treatment-mutant_Rep2_ATH1.cel |
| Treatment: The Arabidopsis were treated with 20 micro M trans-zeatin by spraying for 1h |
| Sakakibara_2-9_TZ-treatment-mutant_Rep3_ATH1 | 20787 | | roots | N598604, N554752 | Sakakibara_2-9_TZ-treatment-mutant_Rep3_ATH1.cel |
| Treatment: The Arabidopsis were treated with 20 micro M trans-zeatin by spraying for 1h |
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.