Experiment: | 390 |
Title: | Transciptome differences between two vitamin C-deficient mutants and the wild type |
Date: | 2007-11-19 |
Description: | The Arabidopsis mutant vtc2 is vitamin C-deficient and high light sensitive ((Mller-Moul et al., 2002. Plant Physiol 128: 970-977; Mller-Moul© et al., 2003. Plant Physiol 133: 748-760). Even though the VTC2 gene has been cloned (Jander et al., 2002. Plant Physiol 129: 440-450), its function is still unclear. The goal of this experiment is to learn more about the function of the VTC2 gene by determining differences in the transcriptome of the wild type and the vtc2-1 mutant. In order to differentiate between VTC2 gene specific differences and vitamin C-induced differences, a second vitamin C-deficient mutant vtc1-1 (Conklin et al., 1996. PNAS 93: 9970-9974) is included in the experiment. The plants will be grown on agar plates in a growth chamber with a 16 hour light (about 40 µmol photons m²s¹)/8 hour dark regime at 20°C for 14 days to stage 1.04. Whole plants will be collected and total RNA will be isolated using the Qiagen Rneasy plant mini kit (Qiagen, Hilden, Germany). |
ftp Link: | ftp Link |
Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
---|---|---|---|---|---|
Mueller-Moule_1-1_Col-0_Rep1_ATH1 | 20650 | Whole plant | Mueller-Moule_1-1_Col-0_Rep1_ATH1.CEL | ||
Mueller-Moule_1-2_Col-0_Rep2_ATH1 | 20651 | Whole plant | Mueller-Moule_1-2_Col-0_Rep2_ATH1.CEL | ||
Mueller-Moule_1-3_Col-0_Rep3_ATH1 | 20652 | Whole plant | Mueller-Moule_1-3_Col-0_Rep3_ATH1.CEL | ||
Mueller-Moule_1-4_vtc1_Rep1_ATH1 | 20653 | Whole plant | N8326 | Mueller-Moule_1-4_vtc1_Rep1_ATH1.CEL | |
Mueller-Moule_1-5_vtc1_Rep2_ATH1 | 20654 | Whole plant | N8326 | Mueller-Moule_1-5_vtc1_Rep2_ATH1.CEL | |
Mueller-Moule_1-6_vtc1_Rep3_ATH1 | 20655 | Whole plant | N8326 | Mueller-Moule_1-6_vtc1_Rep3_ATH1.CEL | |
Mueller-Moule_1-7_vtc2_Rep1_ATH1 | 20656 | Col-0 | Whole plant | Mueller-Moule_1-7_vtc2_Rep1_ATH1.CEL | |
Mueller-Moule_1-8_vtc2_Rep2_ATH1 | 20657 | Col-0 | Whole plant | Mueller-Moule_1-8_vtc2_Rep2_ATH1.CEL | |
Mueller-Moule_1-9_vtc2_Rep3_ATH1 | 20658 | Col-0 | Whole plant | Mueller-Moule_1-9_vtc2_Rep3_ATH1.CEL |