NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:385
Title:GA Treatment of Arabidopsis Root Tips
Date:2006-05-24
Description:The plant hormone gibberellin represents an important regulator of growth and development. Early transcriptional events controlled by GA are not well characterised. Previous microarray studies have identified genes responsive to GA treatment in the whole seedling. The whole seedling represents many tissues where subtle effects of GA treatment in specific tissues may be masked. When treated with GA an effect on the growth rate of roots was observed. More specifically the shorter root of a GA deficient plant can be rescued to wild type length by the application of GA. This experiment was designed to identify GA regulated genes in the root tips of Arabidopsis. The use of a GA deficient mutant provides a greater potential to identify genes responding to GA treatment. Root tips are ideally suited for the quick uptake of the hormone treatment. There will be two biological replicates which will each consist of a control treatment at 0 minutes and 2 hours as well as the experimental GA treated 2 hour time point. This system provides an opportunity to compare gene expression between treated and non-treated root tips and allow the identification of early GA responsive genes.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Griffiths_1-1_AtCON0.1_Rep1_ATH14817root tips Griffiths_1-1_AtCON0.1_Rep1_ATH1.CEL
Treatment: Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours.
Griffiths_1-2_AtCON120.1_Rep1_ATH14818root tips Griffiths_1-2_AtCON120.1_Rep1_ATH1.CEL
Treatment: Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours.
Griffiths_1-3_AtGA120.1_Rep1_ATH14819Col-0root tips Griffiths_1-3_AtGA120.1_Rep1_ATH1.CEL
Treatment: Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours.
Griffiths_1-4_AtCON0.2_Rep2_ATH14820root tips Griffiths_1-4_AtCON0.2_Rep2_ATH1.CEL
Treatment: Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours.
Griffiths_1-5_AtCON120.2_Rep2_ATH14821root tips Griffiths_1-5_AtCON120.2_Rep2_ATH1.CEL
Treatment: Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours.
Griffiths_1-6_AtGA120.2_Rep2_ATH14822Col-0root tips Griffiths_1-6_AtGA120.2_Rep2_ATH1.CEL
Treatment: Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours.