NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:381
Title:Growth of suspension-cultured cells
Date:2006-06-20
Description:The growth of Arabidopsis cell cultures following their sub-culture into fresh media follows standard growth kinetics of a period of exponential increase associated with high rate of cell division, followed by a slowing of the rate of increase as cells approach stationary phase. For the analysis described here, MM2d cells were subcultured into fresh MSS-medium and samples were taken at day 1, day3, day 5 and day7. We have carried out transcriptional profiling analysis with the aim to follow growth stage specific gene expression during unperturbed growth using the near full genome ATH1 arrays (Menges et al., 2003).Journal Absract:(Plant Molecular Biology: 53, 2003)Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during synchronous cell cycle re-entry and during synchronous cell cycle progression provides a unique integrated view of gene expression responses in a higher-plant system. Particularly striking is that expression of over 14 000 genes belonging to all defined categories can be reliably detected, suggesting that integrated and comparative analysis of data sets derived from transcript profiling of cultures is a powerful approach to identify candidate components involved in a wide range of biological processes. Combinatorial analysis of independent cell cycle synchrony methods allows the identification of genes that are apparently cell-cycle-regulated but are most likely responding to the induction of synchrony. We thus present an integrated genome-wide view of the transcriptional profile of a plant suspension culture and identify a refined set of 1082 cell cycle regulated genes largely independent of synchrony method.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Murray_3-1_D1-GROWTH_Rep1_ATH14796cell suspension Murray_3-1_D1-GROWTH_Rep1_ATH1.cel
Treatment: An aliquot of 5 ml of an early stationary phase MM2d cell suspension (7 days after previous subculture) was inoculated into 100 ml fresh MSS-medium (MS-salt, 3% sucrose, 0.5mg/l NAA, 0.05mg/l kinetin). Cells were incubated under continuous darkness by rotating at 130 rpm at a temperature of 27C and samples were taken every two days for analysis. The D1 sample was taken at day 1 after subculture into fresh medium (exp. Variable; time course).
Murray_3-2_D3-GROWTH_Rep1_ATH14797cell suspension Murray_3-2_D3-GROWTH_Rep1_ATH1.cel
Treatment: An aliquot of 5 ml of an early stationary phase MM2d cell suspension (7 days after previous subculture) was inoculated into 100 ml fresh MSS-medium (MS-salt, 3% sucrose, 0.5mg/l NAA, 0.05mg/l kinetin). Cells were incubated under continuous darkness by rotating at 130 rpm at a temperature of 27C and samples were taken every two days for analysis. The D3 sample was taken at day 3 after subculture into fresh medium (exp. variable; time course).
Murray_3-3_D5-GROWTH_Rep1_ATH14798cell suspension Murray_3-3_D5-GROWTH_Rep1_ATH1.cel
Treatment: An aliquot of 5 ml of an early stationary phase MM2d cell suspension (7 days after previous subculture) was inoculated into 100 ml fresh MSS-medium (MS-salt, 3% sucrose, 0.5mg/l NAA, 0.05mg/l kinetin). Cells were incubated under continuous darkness by rotating at 130 rpm at a temperature of 27C and samples were taken every two days for analysis. The D5 sample was taken at day 5 after subculture into fresh medium (exp. variable; time course).
Murray_3-4_D7-GROWTH_Rep1_ATH14799cell suspension Murray_3-4_D7-GROWTH_Rep1_ATH1.cel
Treatment: An aliquot of 5 ml of an early stationary phase MM2d cell suspension (7 days after previous subculture) was inoculated into 100 ml fresh MSS-medium (MS-salt, 3% sucrose, 0.5mg/l NAA, 0.05mg/l kinetin). Cells were incubated under continuous darkness by rotating at 130 rpm at a temperature of 27C and samples were taken every two days for analysis. The D7 sample was taken at day 7 after subculture into fresh medium (exp. variable; time course).