NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:379
Title:Identification of genes regulated by NMD in Arabidopsis
Date:2007-07-11
Description:The nonsense-mediated mRNA decay pathway (NMD) is a quality control mechanism that detects and degrades mRNAs that contain premature translation termination codons (PTCs), thus preventing the production of potentially harmful truncated proteins. NMD has been studied in a number of organisms including humans, yeast, Drosophila, C. elegans and recently Arabidopsis. Recently the NMD process has been shown to have a wider significance in regulating the levels of mRNA expression for a wide range of genes in yeast and Drosophila. Several genes have been found to be required for NMD in a variety of organisms, including UPF1 and UPF3. We have identified and characterised a number of alleles of UPF1 and UPF3 in Arabidopsis that are defective in NMD. By doing a transcriptomic analysis of two NMD mutants, upf1-1 and upf3-1, and wild type plants we aim to identify the subset of genes that are co-regulated by UPF1 and UPF3 and, therefore, by NMD in Arabidopsis. RNA will be extracted from 17 day-old mutant and wild type seedlings grown at 22-24 C under constant light.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Arciga-Reyes_1-1_wildtype_Rep1_ATH14782whole plant Arciga-Reyes_1-1_wildtype_Rep1_ATH1.CEL
Arciga-Reyes_1-2_wildtype_Rep2_ATH14783whole plant Arciga-Reyes_1-2_wildtype_Rep2_ATH1.CEL
Arciga-Reyes_1-3_wildtype_Rep3_ATH14784whole plant Arciga-Reyes_1-3_wildtype_Rep3_ATH1.CEL
Arciga-Reyes_1-4_upf1_Rep1_ATH14785whole plant N612922Arciga-Reyes_1-4_upf1_Rep1_ATH1.CEL
Arciga-Reyes_1-5_upf1_Rep2_ATH14786whole plant N612922Arciga-Reyes_1-5_upf1_Rep2_ATH1.CEL
Arciga-Reyes_1-6_upf3_Rep1_ATH14787whole plant N525175Arciga-Reyes_1-6_upf3_Rep1_ATH1.CEL
Arciga-Reyes_1-7_upf3_Rep2_ATH14788whole plant N525175Arciga-Reyes_1-7_upf3_Rep2_ATH1.CEL