Experiment: | 379 |
Title: | Identification of genes regulated by NMD in Arabidopsis |
Date: | 2007-07-11 |
Description: | The nonsense-mediated mRNA decay pathway (NMD) is a quality control mechanism that detects and degrades mRNAs that contain premature translation termination codons (PTCs), thus preventing the production of potentially harmful truncated proteins. NMD has been studied in a number of organisms including humans, yeast, Drosophila, C. elegans and recently Arabidopsis. Recently the NMD process has been shown to have a wider significance in regulating the levels of mRNA expression for a wide range of genes in yeast and Drosophila. Several genes have been found to be required for NMD in a variety of organisms, including UPF1 and UPF3. We have identified and characterised a number of alleles of UPF1 and UPF3 in Arabidopsis that are defective in NMD. By doing a transcriptomic analysis of two NMD mutants, upf1-1 and upf3-1, and wild type plants we aim to identify the subset of genes that are co-regulated by UPF1 and UPF3 and, therefore, by NMD in Arabidopsis. RNA will be extracted from 17 day-old mutant and wild type seedlings grown at 22-24 C under constant light. |
ftp Link: | ftp Link |
Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
---|---|---|---|---|---|
Arciga-Reyes_1-1_wildtype_Rep1_ATH1 | 4782 | whole plant | Arciga-Reyes_1-1_wildtype_Rep1_ATH1.CEL | ||
Arciga-Reyes_1-2_wildtype_Rep2_ATH1 | 4783 | whole plant | Arciga-Reyes_1-2_wildtype_Rep2_ATH1.CEL | ||
Arciga-Reyes_1-3_wildtype_Rep3_ATH1 | 4784 | whole plant | Arciga-Reyes_1-3_wildtype_Rep3_ATH1.CEL | ||
Arciga-Reyes_1-4_upf1_Rep1_ATH1 | 4785 | whole plant | N612922 | Arciga-Reyes_1-4_upf1_Rep1_ATH1.CEL | |
Arciga-Reyes_1-5_upf1_Rep2_ATH1 | 4786 | whole plant | N612922 | Arciga-Reyes_1-5_upf1_Rep2_ATH1.CEL | |
Arciga-Reyes_1-6_upf3_Rep1_ATH1 | 4787 | whole plant | N525175 | Arciga-Reyes_1-6_upf3_Rep1_ATH1.CEL | |
Arciga-Reyes_1-7_upf3_Rep2_ATH1 | 4788 | whole plant | N525175 | Arciga-Reyes_1-7_upf3_Rep2_ATH1.CEL |