NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	370
Title:	Identification of AtATM dependent X-ray inducible transcript
Date:	2007-07-11
Description:	ATM plays an important role in the response of plants to forms of DNA damage that cause DNA double strand breaks. Arabidopsis ATM has been shown to be important for the transcriptional induction of several DNA repair genes including AtRAD51 (Garcia et al 2003 Plant Cell 15, 119-132). In our experiment we compare the transcript profiles of wild type and mutant plants exposed to 10Gy X-rays (dose rate ~0.8 Gy/min). Plants will be grown on 0.5*MS + 1%sucrose at 22degC under short (9h) day conditions and treated after 5h light. We will irradiate wild type (Col-0) and atatm-3 mutant (N589805) seedlings at growth stage 1.06 and harvest tissue 1h post irradiation. RNA will be isolated using the SV RNA isolation kit (Promega). Comparison of the transcript profiles will then allow AtATM-dependent X-ray inducible transcripts to be identified.
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Tissue	Stock Code	Cel File
West_2-1_Col0_Rep1_ATH1	4555	whole plant	N1092	West_2-1_Col0_Rep1_ATH1.CEL
West_2-1_Col0_Rep1_ATH1	Treatment: Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment.
West_2-2_Col0_Rep2_ATH1	4556	whole plant	N1092	West_2-2_Col0_Rep2_ATH1.CEL
West_2-2_Col0_Rep2_ATH1	Treatment: Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment.
West_2-3_Col0_Rep3_ATH1	4557	whole plant	N1092	West_2-3_Col0_Rep3_ATH1.CEL
West_2-3_Col0_Rep3_ATH1	Treatment: Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment.
West_2-4_atm_Rep1_ATH1	4558	whole plant	N589805	West_2-4_atm_Rep1_ATH1.CEL
West_2-4_atm_Rep1_ATH1	Treatment: Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment.
West_2-5_atm_Rep2_ATH1	4559	whole plant	N589805	West_2-5_atm_Rep2_ATH1.CEL
West_2-5_atm_Rep2_ATH1	Treatment: Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment.
West_2-6_atm_Rep3_ATH1	4560	whole plant	N589805	West_2-6_atm_Rep3_ATH1.CEL
West_2-6_atm_Rep3_ATH1	Treatment: Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment.