NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:364
Title:Transcript profiling of auxin/cytokinin crosstalk in the Arabidopsis primary root apex
Date:2007-02-03
Description:AtIPT8/pga22 seedlings (gain of function mutant in Ws background; Sun et al. 2003, Plant Physiology 131, pp167-176) were grown on vertical plates for 7 days in LD. The seedlings were then incubated directly on the plate with medium containing 5 mM 17-beta-estradiol (for induction of the IPT8 gene) or 5 mM trans-zeatin for 12 and 24 h. 5 mm primary root tips were harvested from the seedlings and pooled for microarray analysis. Total RNA was isolated from the samples with the RNeasy Plant Mini Kit from Qiagen.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Ljung_1-10_K24h_Rep2_ATH14376WassilewskijaPrimary root tip (5 mm) Ljung_1-10_K24h_Rep2_ATH1.CEL
Treatment: Control seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) for 24 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperatuse etc.) were also the same.
Ljung_1-11_E24h_Rep1_ATH14377WassilewskijaPrimary root tip (5 mm) Ljung_1-11_E24h_Rep1_ATH1.CEL
Treatment: Estradiol treated seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) containing 5 micromolar 17-beta-estradiol for 24 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same.
Ljung_1-12_E24h_Rep2_ATH14378WassilewskijaPrimary root tip (5 mm) Ljung_1-12_E24h_Rep2_ATH1.CEL
Treatment: Estradiol treated seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) containing 5 micromolar 17-beta-estradiol for 24 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same.
Ljung_1-13_Z24h_Rep1_ATH14379WassilewskijaPrimary root tip (5 mm) Ljung_1-13_Z24h_Rep1_ATH1.CEL
Treatment: Zeatin treted seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) containing 5 micromolar trans-zeatin for 24 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same.
Ljung_1-14_Z24h_Rep2_ATH14380WassilewskijaPrimary root tip (5 mm) Ljung_1-14_Z24h_Rep2_ATH1.CEL
Treatment: Zeatin treated seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) containing 5 micromolar trans-zeatin for 24 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same.
Ljung_1-1_K12h_Rep1_ATH14367WassilewskijaPrimary root tip (5 mm) Ljung_1-1_K12h_Rep1_ATH1.CEL
Treatment: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same. Control samples: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) for 12 h.
Ljung_1-2_K12h_Rep2_ATH14368WassilewskijaPrimary root tip (5 mm) Ljung_1-2_K12h_Rep2_ATH1.CEL
Treatment: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same. Control samples: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) for 12 h.
Ljung_1-3_K12h_Rep3_ATH14369WassilewskijaPrimary root tip (5 mm) Ljung_1-3_K12h_Rep3_ATH1.CEL
Treatment: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same. Control samples: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) for 12 h.
Ljung_1-4_E12h_Rep1_ATH14370WassilewskijaPrimary root tip (5 mm) Ljung_1-4_E12h_Rep1_ATH1.CEL
Treatment: Estradiol treated seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) containing 5 micromolar 17-beta-estradiol for 12 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same.
Ljung_1-5_E12h_Rep2_ATH14371WassilewskijaPrimary root tip (5 mm) Ljung_1-5_E12h_Rep2_ATH1.CEL
Treatment: Estradiol treated seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) containing 5 micromolar 17-beta-estradiol for 12 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same.
Ljung_1-6_E12h_Rep3_ATH14372WassilewskijaPrimary root tip (5 mm) Ljung_1-6_E12h_Rep3_ATH1.CEL
Treatment: Estradiol treated seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) containing 5 micromolar 17-beta-estradiol for 12 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same.
Ljung_1-7_Z12h_Rep1_ATH14373WassilewskijaPrimary root tip (5 mm) Ljung_1-7_Z12h_Rep1_ATH1.CEL
Treatment: Zeatin treated seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) containing 5 micromolar trans-zeatin for 12 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same.
Ljung_1-8_Z12h_Rep2_ATH14374WassilewskijaPrimary root tip (5 mm) Ljung_1-8_Z12h_Rep2_ATH1.CEL
Treatment: Zeatin treated seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) containing 5 micromolar trans-zeatin for 12 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperature etc.) were also the same.
Ljung_1-9_K24h_Rep1_ATH14375Wassilewskija Ljung_1-9_K24h_Rep1_ATH1.CEL
Treatment: Control seedlings: these seedlings were treated with liquid medium (1 x MS, 1 % sucrose, pH 5.7) for 24 h. General proceadure: The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5 micro-molar 17-beta-estradiol or 5 micro-molar trans-zeatin for 12 and 24 h. The plates were changed from vertical to horisontal position, and 20 ml medium was poured on to the plate. The composition of the liquid medium was the same as in the agar plates and other growth conditions (light, temperatuse etc.) were also the same.