NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:361
Title:In vitro tracheary element transdifferentiation of Col-0 suspension cells.
Date:2006-04-20
Description:This experiment was annotated by TAIR (http://arabidopsis.org).Col-0 suspension cells provided by Dr. C. Koncz and Dr. M. Umeda. 15 ml of Arabidopsis Col-0 suspension cells (Mathur et al. 1998) was transferred to 35 ml of a fresh modified Murashige and Skoog (MS) medium supplemented with 1 ug /ml 2,4-dichlorphenoxyacetic acid and 3% sucrose every 7 day, and cultured on a rotary shaker at 120 rpm in the dark at 22 C for subculture. For xylem vessel element induction, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3, and cultured as described above. The frequency of xylem vessel element formation was calculated as the proportion of xylem vessel elements to the number of living cells and the vessel elements.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Fukuda_1-10_8B_Rep2_ATH14288Col-0 suspension cells N1092Fukuda_1-10_8B_Rep2_ATH1.cel
Treatment: For in vitro tracheary element (xylem vessel element) transdifferentiation, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3. Cells were collected by centrigugation after 8 days of culture.
Fukuda_1-11_10A_Rep1_ATH14289Col-0 suspension cells N1092Fukuda_1-11_10A_Rep1_ATH1.cel
Treatment: For in vitro tracheary element (xylem vessel element) transdifferentiation, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3. Cells were collected by centrigugation after 10 days of culture.
Fukuda_1-12_10B_Rep2_ATH14290Col-0 suspension cells N1092Fukuda_1-12_10B_Rep2_ATH1.cel
Treatment: For in vitro tracheary element (xylem vessel element) transdifferentiation, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3. Cells were collected by centrigugation after 10 days of culture.
Fukuda_1-1_0A_Rep1_ATH14279Col-0 suspension cells N1092Fukuda_1-1_0A_Rep1_ATH1.cel
Treatment: no treatment
Fukuda_1-2_0B_Rep2_ATH14280Col-0 suspension cells N1092Fukuda_1-2_0B_Rep2_ATH1.cel
Treatment: no treatment
Fukuda_1-3_2A_Rep1_ATH14281Col-0 suspension cells N1092Fukuda_1-3_2A_Rep1_ATH1.cel
Treatment: For in vitro tracheary element (xylem vessel element) transdifferentiation, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3. Cells were collected by centrigugation after 2 days of culture.
Fukuda_1-4_2B_Rep2_ATH14282Col-0 suspension cells N1092Fukuda_1-4_2B_Rep2_ATH1.cel
Treatment: For in vitro tracheary element (xylem vessel element) transdifferentiation, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3. Cells were collected by centrigugation after 2 days of culture.
Fukuda_1-5_4A_Rep1_ATH14283Col-0 suspension cells N1092Fukuda_1-5_4A_Rep1_ATH1.cel
Treatment: For in vitro tracheary element (xylem vessel element) transdifferentiation, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3. Cells were collected by centrigugation after 4 days of culture.
Fukuda_1-6_4B_Rep2_ATH14284Col-0 suspension cells N1092Fukuda_1-6_4B_Rep2_ATH1.cel
Treatment: For in vitro tracheary element (xylem vessel element) transdifferentiation, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3. Cells were collected by centrigugation after 4 days of culture.
Fukuda_1-7_6A_Rep1_ATH14285Col-0 suspension cells N1092Fukuda_1-7_6A_Rep1_ATH1.cel
Treatment: For in vitro tracheary element (xylem vessel element) transdifferentiation, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3. Cells were collected by centrigugation after 6 days of culture.
Fukuda_1-8_6B_Rep2_ATH14286Col-0 suspension cells N1092Fukuda_1-8_6B_Rep2_ATH1.cel
Treatment: For in vitro tracheary element (xylem vessel element) transdifferentiation, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3. Cells were collected by centrigugation after 6 days of culture.
Fukuda_1-9_8A_Rep1_ATH14287Col-0 suspension cells N1092Fukuda_1-9_8A_Rep1_ATH1.cel
Treatment: For in vitro tracheary element (xylem vessel element) transdifferentiation, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3. Cells were collected by centrigugation after 8 days of culture.