NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:345
Title:AvrPtoB effector function
Date:2009-05-18
Description:In planta expression of the bacterial effector AvrPtoB induces susceptibility to bacterial invasion. We have used stable transgenic lines of ecotype Nd expressing avrPtoB under the control of a dexamethosone inducible promoter.Expressing lines given no treatment or Dex induction are left for 6h before challenge with suspensions of Pseudomonas syringae pv tomato strain DC3000 hrpA mutant or a mock inoculation with MgCl2. Tissues were sampled 0, 2, 4 and 12h after bacterial inoculation. Preliminary northern analyses indicated a specific suppression of defence-related transcripts by AvrPtoB. The array will provide a global picture.In a related experiment we examine the effect of AvrptoB when delivered by bacteria into arabidopsis. Triplicate samples are studied at each time point. Leaves from half the plants were treated with a solution of dexamethasone (6µM in water containing 0.02% Silwet). Six hours later (our start time in this time course), Dex treated and untreated leaves were either harvested (t=0) or infiltrated with either 10 mM MgCl2 or a suspension of Pseudomonas syringae pv. tomato hrpA mutant (DC3000; 1.9x108 colony forming units/ml) in 10mM MgCl2.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Torres_2-10_BH4.1_Rep1_ATH14052Leaves Torres_2-10_BH4.1_Rep1_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2 at the start time of this time course. Four hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-11_BH4.2_Rep2_ATH14053Leaves Torres_2-11_BH4.2_Rep2_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2 at the start time of this time course. Four hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-12_BH4.3_Rep3_ATH14054Leaves Torres_2-12_BH4.3_Rep3_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2 at the start time of this time course. Four hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-13_BH12.1_Rep1_ATH14055Leaves Torres_2-13_BH12.1_Rep1_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2 at the start time of this time course. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-14_BH12.2_Rep2_ATH14056Leaves Torres_2-14_BH12.2_Rep2_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2 at the start time of this time course. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-15_BH12.3_Rep3_ATH14057Leaves Torres_2-15_BH12.3_Rep3_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2 at the start time of this time course. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-16_DM2.1_Rep1_ATH14058Leaves Torres_2-16_DM2.1_Rep1_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing 10 mM MgCl2. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-17_DM2.2_Rep2_ATH14059Leaves Torres_2-17_DM2.2_Rep2_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing 10 mM MgCl2. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-18_DM2.3_Rep3_ATH14060Leaves Torres_2-18_DM2.3_Rep3_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing 10 mM MgCl2. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-19_DH2.1_Rep1_ATH14061Leaves Torres_2-19_DH2.1_Rep1_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing 10 mM MgCl2. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-1_B0.1_Rep1_ATH14043Leaves Torres_2-1_B0.1_Rep1_ATH1.CEL
Torres_2-20_DH2.2_Rep2_ATH14062Leaves Torres_2-20_DH2.2_Rep2_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing 10 mM MgCl2. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-21_DH2.3_Rep3_ATH14063Leaves Torres_2-21_DH2.3_Rep3_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing 10 mM MgCl2. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-22_DH4.1_Rep1_ATH14064Leaves Torres_2-22_DH4.1_Rep1_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2. Four hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-23_DH4.2_Rep2_ATH14065Leaves Torres_2-23_DH4.2_Rep2_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2. Four hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-24_DH4.3_Rep3_ATH14066Leaves Torres_2-24_DH4.3_Rep3_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2. Four hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-25_DH12.1_Rep1_ATH14067Leaves Torres_2-25_DH12.1_Rep1_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-26_DH12.2_Rep2_ATH14068Leaves Torres_2-26_DH12.2_Rep2_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-27_DH12.3_Rep3_ATH14069Leaves Torres_2-27_DH12.3_Rep3_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet) and six hours later (start time of this time course) they were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-28_DO.1_Rep1_ATH14070Leaves Torres_2-28_DO.1_Rep1_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet). Six hours later (start time of this time course), leaves were harvested and snap frozen in liquid N2.
Torres_2-29_DO.2_Rep2_ATH14071Leaves Torres_2-29_DO.2_Rep2_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet). Six hours later (start time of this time course), leaves were harvested and snap frozen in liquid N2.
Torres_2-2_B0.2_Rep2_ATH14044Leaves Torres_2-2_B0.2_Rep2_ATH1.CEL
Torres_2-30_DO.3_Rep3_ATH14072Leaves Torres_2-30_DO.3_Rep3_ATH1.CEL
Treatment: Leaves were brushed with a dexamethasone solution (6µM in water containing 0.02% Silwet). Six hours later (start time of this time course), leaves were harvested and snap frozen in liquid N2.
Torres_2-31_W6012.1_Rep1_ATH14073 N1638Torres_2-31_W6012.1_Rep1_ATH1.CEL
Treatment: Leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of RW60 carrying the plasmid pDSK600 ( 2.3 x108 colony forming units/ml) in 10mM MgCl2. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-32_W6012.2_Rep2_ATH14074 N1638Torres_2-32_W6012.2_Rep2_ATH1.CEL
Treatment: Leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of RW60 carrying the plasmid pDSK600 ( 2.3 x108 colony forming units/ml) in 10mM MgCl2. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-33_W6012.3_Rep3_ATH14075 N1638Torres_2-33_W6012.3_Rep3_ATH1.CEL
Treatment: Leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of RW60 carrying the plasmid pDSK600 ( 2.3 x108 colony forming units/ml) in 10mM MgCl2. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-34_W60B12.1_Rep1_ATH14076 N1638Torres_2-34_W60B12.1_Rep1_ATH1.CEL
Treatment: Leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of RW60 carrying the plasmid pDSK600-avrPtoB ( 2.3 x108 colony forming units/ml) in 10mM MgCl2. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-35_W60B12.2_Rep2_ATH14077 N1638Torres_2-35_W60B12.2_Rep2_ATH1.CEL
Treatment: Leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of RW60 carrying the plasmid pDSK600-avrPtoB ( 2.3 x108 colony forming units/ml) in 10mM MgCl2. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-36_W60B12.3_Rep3_ATH14078 N1638Torres_2-36_W60B12.3_Rep3_ATH1.CEL
Treatment: Leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of RW60 carrying the plasmid pDSK600-avrPtoB ( 2.3 x108 colony forming units/ml) in 10mM MgCl2. Twelve hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-37_W0.1_Rep1_ATH14079 N1638Torres_2-37_W0.1_Rep1_ATH1.CEL
Torres_2-38_W0.2_Rep2_ATH14080 N1638Torres_2-38_W0.2_Rep2_ATH1.CEL
Torres_2-39_W0.3_Rep3_ATH14081 N1638Torres_2-39_W0.3_Rep3_ATH1.CEL
Torres_2-3_B0.3_Rep3_ATH14045Leaves Torres_2-3_B0.3_Rep3_ATH1.CEL
Torres_2-4_B2.1_Rep1_ATH14046Leaves Torres_2-4_B2.1_Rep1_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing 10 mM MgCl2 at the start time of this time course. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-5_B2.2_Rep2_ATH14047Leaves Torres_2-5_B2.2_Rep2_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing 10 mM MgCl2 at the start time of this time course. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-6_B2.3_Rep3_ATH14048Leaves Torres_2-6_B2.3_Rep3_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing 10 mM MgCl2 at the start time of this time course. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-7_BH2.1_Rep1_ATH14049Leaves Torres_2-7_BH2.1_Rep1_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2 at the start time of this time course. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-8_BH2.2_Rep2_ATH14050Leaves Torres_2-8_BH2.2_Rep2_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2 at the start time of this time course. Two hours later, leaves were harvested and snap frozen in liquid N2.
Torres_2-9_BH2.3_Rep3_ATH14051Leaves Torres_2-9_BH2.3_Rep3_ATH1.CEL
Treatment: Untreated leaves were infiltrated on the abaxial surface with a needle-less syringe containing a suspension of DC3000 hrpA mutant (1.9x108 colony forming units/ml) in 10mM MgCl2 at the start time of this time course. Two hours later, leaves were harvested and snap frozen in liquid N2.