| Description: | The Arabidopsis GATA transcription factor family has 30 members and the biological function of most of them is poorly understood. Homozygous T-DNA insertion lines for 23 of the 30 members were identified and analyzed. Genetic screening of the insertion lines on a defined growth condition revealed one line with an altered phenotype, while the rest showed no obvious change. This line, SALK_001778, has a T-DNA insertion in the second exon of At5g56860 which prevents the expression of the GATA domain. Genetic analysis of the mutant demonstrated that the phenotypic change is caused by a single gene effect and is recessive to the wild type allele. In wild type plants, the expression of At5g56860 is shoot-specific, transcribed at an early stage of development and is inducible by nitrate. Loss of its expression in the loss-of-function mutant plants resulted in reduced chlorophyll levels. A transcript profiling experiment revealed that a considerable proportion of genes down-regulated in the loss-of-function mutants are involved in carbon metabolism and At5g56860 is thus designated GNC (GATA, Nitrate-inducible, Carbon metabolism-involved). gnc mutants with no GNC expression are more sensitive to exogenous glucose and two hexose transporter genes, with a possible connection to glucose signaling, are significantly down-regulated, while GNC over-expressing transgenic plants up-regulate their expression and are less sensitive to exogenous glucose. These observations suggest a function for GNC in regulating carbon and nitrogen metabolism.reference: Yong-Mei Bi et al, Plant J. in press |
NASCArrays Information at The BAR
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