NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Transcriptional profiling in gnc mutant and wild type plants
Description:The Arabidopsis GATA transcription factor family has 30 members and the biological function of most of them is poorly understood. Homozygous T-DNA insertion lines for 23 of the 30 members were identified and analyzed. Genetic screening of the insertion lines on a defined growth condition revealed one line with an altered phenotype, while the rest showed no obvious change. This line, SALK_001778, has a T-DNA insertion in the second exon of At5g56860 which prevents the expression of the GATA domain. Genetic analysis of the mutant demonstrated that the phenotypic change is caused by a single gene effect and is recessive to the wild type allele. In wild type plants, the expression of At5g56860 is shoot-specific, transcribed at an early stage of development and is inducible by nitrate. Loss of its expression in the loss-of-function mutant plants resulted in reduced chlorophyll levels. A transcript profiling experiment revealed that a considerable proportion of genes down-regulated in the loss-of-function mutants are involved in carbon metabolism and At5g56860 is thus designated GNC (GATA, Nitrate-inducible, Carbon metabolism-involved). gnc mutants with no GNC expression are more sensitive to exogenous glucose and two hexose transporter genes, with a possible connection to glucose signaling, are significantly down-regulated, while GNC over-expressing transgenic plants up-regulate their expression and are less sensitive to exogenous glucose. These observations suggest a function for GNC in regulating carbon and nitrogen metabolism.reference: Yong-Mei Bi et al, Plant J. in press
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Bi_1-1_wt1_Rep1_SyngentaChip4018leaves N60000Bi_1-1_wt1_Rep1_ATH1.CEL
Bi_1-2_mutant1_Rep1_SyngentaChip4019Col-0 (Columbia, CS60000)leaves N501778Bi_1-2_mutant1_Rep1_ATH1.CEL
Bi_1-3_wt2_Rep2_SyngentaChip4020leaves N60000Bi_1-3_wt2_Rep2_ATH1.CEL
Bi_1-4_mutant2_Rep2_SyngentaChip4021Col-0 (Columbia, CS60000)leaves N501778Bi_1-4_mutant2_Rep2_ATH1.CEL
Bi_1-5_wt3_Rep3_SyngentaChip4022leaves N60000Bi_1-5_wt3_Rep3_ATH1.CEL
Bi_1-6_mutant3_Rep3_SyngentaChip4023Col-0 (Columbia, CS60000)leaves N501778Bi_1-6_mutant3_Rep3_ATH1.CEL