NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:341
Title:Nuclear gene expression in response to chloroplast division inhibition
Date:2006-10-26
Description:The aim of this investigation is to analyse the affect on nuclear gene expression of inhibition of chloroplast division. Transgenic Arabidopsis lines (Ler) were generated that contained a chemically inducible promoter (XVE)upstream of AtMinD1. AtMinD1 encodes a product involved in chloroplast division, overexpression of AtMinD1 leads to chloroplast division inhibition. Three treatments were performed: Treatment 1; Empty vector control seedlings (transformed with PER-10 only)were sown onto lehle and sprayed at 13 days old with 17-B-estradiol and were harvested 24 hours later. Treatment 2; seeds were sown on lehle containing inducer and sprayed every 24 hours until harvested at 14 days old.Treatment 3; seeds were sown on lehle plates and sprayed at 13 days old and harvested 24 hours later. For all three treatments seedlings were grown in constant light conditions, tissue was harvested from 14 day old seedlings (4 rosette leaves) and snap frozen in liquid nitrogen betore RNA preparation.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Aldridge_1-1_Control_Rep1_ATH14012Ler-0whole plant Aldridge_1-1_Control_Rep1_ATH1.CEL
Treatment: 13 day old seedlings were sprayed with 2 microMolar 17-B-estradiol 24 hours before tissue was harvested
Aldridge_1-2_Control_Rep2_ATH14013Ler-0whole plant Aldridge_1-2_Control_Rep2_ATH1.CEL
Treatment: 13 day old seedlings were sprayed with 2 microMolar 17-B-estradiol 24 hours before tissue was harvested
Aldridge_1-3_Prolonged-induction_Rep1_ATH14014Ler-1whole plant Aldridge_1-3_Prolonged-induction_Rep1_ATH1.CEL
Treatment: 2 microMolar 17-B-estradiol was added to the growth mediaseedlings were sprayed with 2 microMolar 17-B-estradiol every 24 hours as soon as seeds germinated
Aldridge_1-4_Prolonged-induction_Rep2_ATH14015Ler-1whole plant Aldridge_1-4_Prolonged-induction_Rep2_ATH1.CEL
Treatment: 2 microMolar 17-B-estradiol was added to the growth mediaseedlings were sprayed with 2 microMolar 17-B-estradiol every 24 hours as soon as seeds germinated
Aldridge_1-5_Temporal-induction_Rep1_ATH14016Ler-1whole plant Aldridge_1-5_Temporal-induction_Rep1_ATH1.CEL
Treatment: 13 day old seedlings were sprayed with 2 microMolar 17-B-estradiol 24 hours before tissue was harvested
Aldridge_1-6_Temporal-induction_Rep2_ATH14017Ler-1whole plant Aldridge_1-6_Temporal-induction_Rep2_ATH1.CEL
Treatment: 13 day old seedlings were sprayed with 2 microMolar 17-B-estradiol 24 hours before tissue was harvested