NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:328
Title:Environmental Genomics of Calcicole-calcifuge physiology
Date:2005-11-04
Description:The purpose of this set of arrays is to provide a third replicate to use in data analysis addition to the 2 previous replicates for all samples already generated on this project. Our hypothesis is that adaptation to a calcareous environment will be reflected in altered gene expression including genes encoding transporters, ion channels, transcription factors, etc. To test this hypothesis we grew a laboratory non-calcicole (Col-4) and a laboratory calcicole (Cal-0) ecotypes of A. thaliana at low (1 mM) and high (12.5 mM) rhizospheric Ca2+ and compare the patterns of gene expression by microarray analysis. We then collected from the wild, a putative calcicole ecotype (Elland)and a putative non-calcicole (Penicuik) and grew both at low (1 mM) and high (12.5 mM) rhizospheric Ca2+ and compared expression profiles to the laboratory ecotypes.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Shirras_3-1_LabCalcicole-1mM-CaCl2_Rep1_ATH13837Whole plant Shirras_3-1_LabCalcicole-1mM-CaCl2_Rep1_ATH1.CEL
Treatment: 1 mM CaCl2
Shirras_3-2_LabCalcifuge-1mM-CaCl2_Rep1_ATH13838Whole plant Shirras_3-2_LabCalcifuge-1mM-CaCl2_Rep1_ATH1.CEL
Treatment: 1mM CaCl2
Shirras_3-3_LabCalcicole-12.5mM-CaCl2_Rep1_ATH13839Whole plant Shirras_3-3_LabCalcicole-12.5mM-CaCl2_Rep1_ATH1.CEL
Treatment: 12.5 mM CaCl2
Shirras_3-4_LabCalcifuge-12.5mM-CaCl2_Rep1_ATH13840Whole plant Shirras_3-4_LabCalcifuge-12.5mM-CaCl2_Rep1_ATH1.CEL
Treatment: 12.5 mM CaCl2
Shirras_3-5_WildEcotype-low-soil-pH-1mM-CaCl2_Rep1_ATH13841Whole plant Shirras_3-5_WildEcotype-low-soil-pH-1mM-CaCl2_Rep1_ATH1.CEL
Treatment: 1mM CaCl2
Shirras_3-6_WildEcotype-high-soil-pH-1mM-CaCl2_Rep1_ATH13842Whole plant Shirras_3-6_WildEcotype-high-soil-pH-1mM-CaCl2_Rep1_ATH1.CEL
Treatment: 1 mM CaCl2
Shirras_3-7_WildEcotype-low-soil-pH-12.5mM-CaCl2_Rep1_ATH13843Whole plant Shirras_3-7_WildEcotype-low-soil-pH-12.5mM-CaCl2_Rep1_ATH1.CEL
Treatment: 12.5 mM CaCl2
Shirras_3-8_WildEcotype-high-soil-pH-12.5mM-CaCl2_Rep1_ATH13844Whole plant Shirras_3-8_WildEcotype-high-soil-pH-12.5mM-CaCl2_Rep1_ATH1.CEL
Treatment: 12.5 mM CaCl2