NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Novel Target Genes Regulated By LEUNIG
Description:Regulation of homeotic gene expression is critical for proper developmental patterns in plants. The Arabidopsis thaliana floral homeotic gene AGAMOUS is regulated by LEUNIG (LUG). Mutations in LUG result in homeotic transformations of floral organ identity, lug mutants also exhibit other defects that are independent of AG, including abnormal carpel and ovule development, reduced female and male fertility, and narrower leaves and floral organs suggesting LUG has a wider role in development. LUG is structurally similar to the transcription co-repressors Tup1 (S. cerevisiae) and Groucho (Drosophila) suggesting developmental pathways regulated by LUG may be controlled by a conserved eukaryotic repression mechanism. We aim to determine novel target genes regulated by LUG using transcriptome analysis. A mutant lug-3 line has been obtained from Dr. Zhongchi Liu. This line was used in the initial studies of LUG in the Meyerowitz lab. Lug-3 is a strong allele in the Lansberg erecta background, caused by a nonsense mutation that results in early termination of the protein and is likely null. Lug-3 mutant exhibit narrow floral organs with staminoid petals and carpelloid sepals, abnormal carpel and ovule development, reduced plant height, increased lateral branching and narrow and smaller leaves. We proposed to use the lug-3 line in elucidating the role of LUG as a potential general regulator of transcription in Arabidopsis. mRNA populations will be isolated from wild type (Ler) and lug-3 plants that have produced inflorescence. This stage of development has been selected for several reasons. Firstly mRNA populations from widest range of tissue types from a single plant can be collected. Secondly, as AGAMOUS, which is involved in the regulation of floral development, is present in the Affymetrix gene chip it will provided an excellent internal control to monitor between chips. Results will demonstrate wheter LUG is likely to have a more global role in regulating transcription, and will identify pathways under the regulation of thisco-repressor like protein.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Gonzalez_1-10_mut-flowers_Rep1_ATH13834flowers Gonzalez_1-10_mut-flowers_REP1_ATH1.CEL
Gonzalez_1-11_mut-flowers_Rep2_ATH13835flowers Gonzalez_1-11_mut-flowers_REP2_ATH1.CEL
Gonzalez_1-12_mut-flowers_Rep3_ATH13836flowers Gonzalez_1-12_mut-flowers_REP3_ATH1.CEL
Gonzalez_1-1_WT-leaves_Rep1_ATH13825matured rosette leaves NW20Gonzalez_1-1_WT-leaves_REP1_ATH1.CEL
Gonzalez_1-2_WT-leaves_Rep2_ATH13826matured rosette leaves NW20Gonzalez_1-2_WT-leaves_REP2_ATH1.CEL
Gonzalez_1-3_WT-leaves_Rep3_ATH13827matured rosette leaves NW20Gonzalez_1-3_WT-leaves_REP3_ATH1.CEL
Gonzalez_1-4_mut-leaves_Rep1_ATH13828matured rosette leaves Gonzalez_1-4_mut-leaves_REP1_ATH1.CEL
Gonzalez_1-5_mut-leaves_Rep2_ATH13829matured rosette leaves Gonzalez_1-5_mut-leaves_REP2_ATH1.CEL
Gonzalez_1-6_mut-leaves_Rep3_ATH13830matured rosette leaves Gonzalez_1-6_mut-leaves_REP3_ATH1.CEL
Gonzalez_1-7_WT-flowers_Rep1_ATH13831flowers Gonzalez_1-7_WT-flowers_REP1_ATH1.CEL
Gonzalez_1-8_WT-flowers_Rep2_ATH13832flowers Gonzalez_1-8_WT-flowers_REP2_ATH1.CEL
Gonzalez_1-9_WT-flowers_Rep3_ATH13833flowers Gonzalez_1-9_WT-flowers_REP3_ATH1.CEL