NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:326
Title:Targets of AtWRKY27 in Arabidopsis leaves
Date:2006-06-29
Description:The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Mukhtar_1-1_XVE-SALK_Rep1_ATH13812 N609290Mukhtar_1-1_XVE-SALK_Rep1_ATH1.CEL
Treatment: Plant were treated for 6 hours with 10mM beta-oestradiol after which RNA was immediately isolated.
Mukhtar_1-2_XVE-ETL_Rep1_ATH13813Col-1 (N3178) Mukhtar_1-2_XVE-ETL_Rep1_ATH1.CEL
Treatment: Plant were treated for 6 hours with 10mM beta-oestradiol after which RNA was immediately isolated.
Mukhtar_1-3_WK27-XVE-SALK_Rep1_ATH13814 N609290Mukhtar_1-3_WK27-XVE-SALK_Rep1_ATH1.CEL
Treatment: Plant were treated for 6 hours with 10mM beta-oestradiol after which RNA was immediately isolated.
Mukhtar_1-4_WK27-XVE-ETL_Rep1_ATH13815Col-1 (N3178) Mukhtar_1-4_WK27-XVE-ETL_Rep1_ATH1.CEL
Treatment: Plant were treated for 6 hours with 10mM beta-oestradiol after which RNA was immediately isolated.