Experiment: | 326 |
Title: | Targets of AtWRKY27 in Arabidopsis leaves |
Date: | 2006-06-29 |
Description: | The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants. |
ftp Link: | ftp Link |
Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
---|---|---|---|---|---|
Mukhtar_1-1_XVE-SALK_Rep1_ATH1 | 3812 | N609290 | Mukhtar_1-1_XVE-SALK_Rep1_ATH1.CEL | ||
Treatment: Plant were treated for 6 hours with 10mM beta-oestradiol after which RNA was immediately isolated. | |||||
Mukhtar_1-2_XVE-ETL_Rep1_ATH1 | 3813 | Col-1 (N3178) | Mukhtar_1-2_XVE-ETL_Rep1_ATH1.CEL | ||
Treatment: Plant were treated for 6 hours with 10mM beta-oestradiol after which RNA was immediately isolated. | |||||
Mukhtar_1-3_WK27-XVE-SALK_Rep1_ATH1 | 3814 | N609290 | Mukhtar_1-3_WK27-XVE-SALK_Rep1_ATH1.CEL | ||
Treatment: Plant were treated for 6 hours with 10mM beta-oestradiol after which RNA was immediately isolated. | |||||
Mukhtar_1-4_WK27-XVE-ETL_Rep1_ATH1 | 3815 | Col-1 (N3178) | Mukhtar_1-4_WK27-XVE-ETL_Rep1_ATH1.CEL | ||
Treatment: Plant were treated for 6 hours with 10mM beta-oestradiol after which RNA was immediately isolated. |