| Description: | ERF domain proteins, a subfamily of ERF(EREBP)/AP2 domain proteins, are members of a novel family of transcription factors unique to plants. A highly conserved DNA binding domain known as the ERF domain is the unique feature of this family. To characterize this novel family of transcription factors in detail, we isolated five cDNAs encoding ERF proteins from Arabidopsis (AtERF1 to AtERF5) and analyzed their DNA binding specificity, transactivation ability and mRNA expression. AtERF1, AtERF2, and AtERF5 functioned as transcriptional activators and specifically bound to the GCC box (AGCCGCC), displaying a very similar in vitro DNA binding specificity. A number of stress inducible genes, such as genes for PDF1 and chitinase, have been shown to contain the GCC box in their promoter regions, and these AtERFs have been implicated to act as transcriptional regulaters for these genes. However, it is not known whether the genes target for AtERF1, AtER2 and AtERF5 are different or not. It is important to analyze the target genes for each AtERF to study the molecular mechanism how transcription factors that possess same DNA binding specificity, recognize their target genes. For this purpose we have prepared transgenic Arabidopsis plant where AtERF1, AtERF2 and AtERF5 are overexpressed and isolated mRNAs form two independent lines of each transgenic plants. We will analyze the regulated genes by AtERF1, AtERF2 and AtERF5 using DNA microarrays. Based on the results, we will study the role of ERF proteins on the regulations of stress related gene expression in more detail |
NASCArrays Information at The BAR
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