Description: | SKS mutant (snakeskin) vs wildtype. Understanding the factors that influence the cell wall properties of plants is of major importance for agriculture and food processing. We have identified an EN transposon-induced mutant known as snakeskin (sks) in which the epidermal cell cell linkages appear to be significantly perturbed. In snakeskin the epidermal cells of the leaves, cotyledons and root peel apart from one another and from the underlying mesophyll cells. As a result of the breakdown of epidermal cell cell linkages in sks there are changes in epidermal cell architecture and distribution. These include alterations in pavement epidermal cell shape, stomatal distribution and trichome morphology. We will describe the characterisation of the snakeskin mutant using a wide range of techniques including reporter genes, cryo-scanning electron microscopy, FTIR, immunogold labelling and mapping of the sks mutation using SSLP markers. |