NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Description:Nitrate and ammonium are critical nutritional sources for higher plants, and they can also serve as signaling molecules to regulate nitrogen metabolism and plant growth. However, little is known about how signal is transmitted from external nitrate or ammonium to plant growth. Arabidopsis CHL1 is a nitrate transporter involved in uptake. Despite the fact that CHL1, a nitrate inducible gene, can contribute up to 80% of nitrate uptake activity under certain conditions, the chl1 deletion mutant plants can grow typically as do the wild type plants in all tested conditions. A CHL1 homolog AtNRT1:2, a constitutive gene, is also known to involve in nitrate uptake. However, different from CHL1, AtNRT1:2, when its expressing is suppressed by anti-sense construct, shows severe growth defect if ammonium but not nitrate is the sole nitrogen source provided. These observations of our lab indicate that CHL1 and AtNRT1:2 can apparently play different roles in regulating plant growth in response to various nitrogen conditions. We value that DNA microarray system may effectively contribute to evaluate and characterize how a signal is transmitted from external ammonium through the activity of AtNRT1:2 to disturb the cellular physiological processes critical for plant growth. The expression patterns of more than ten thousand genes can be systematically and comparatively analyzed by hybridizing to the RNA isolated from anti-AtNRT1:2 plants grown in ammonium versus the RNA isolated from the wild type. This approach will help us to identify candidates for further studies.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File