NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Description:Numerous studies have shown that gene VI of Cauliflower mosaic virus (CaMV) is an important determinant of disease severity in crucifers. For example, studies with chimeric viruses have shown that gene VI is responsible for eliciting chlorosis and mosaic symptoms. Most importantly, it has been shown that transgenic plants that express CaMV gene VI, including Arabidopsis thaliana, exhibit virus-like symptoms. Although CaMV gene VI is biologically important for pathogenesis, little is known about how this gene functions at the molecular level in the virus-host interaction. More traditional methods such as differential display have only provided limited results towards the understanding of disease development in both virus-infected and transgenic plants. The development of microarrays may prove to be a more reliable and powerful tool for an analysis of how viruses affect their hosts. We have created transgenic A. thaliana plants (ecotype Col-0) that express gene VI from two very different strains of CaMV. Plants infected with CaMV strain W260 cause chlorosis and stunting, and transgenic Arabidopsis plants that express W260 gene VI exhibit chlorosis and stunting. Plants infected with CaMV strain D4 exhibit a necrotic flecking symptom (which maps to virus genes other than VI), but no chlorosis. Transgenic lines that express D4 gene VI show no signs of any disease. Thus, these transgenic plants can serve as a good model system for understanding how CaMV gene VI contributes to the formation of specific symptoms because these transgenic lines have nearly identical genetic information, yet the two versions of CaMV gene VI elicit very different responses. We propose two experiments in this application, a total of eight slides. In the first experiment, our highest priority, we will compare two mRNA preparations from symptomatic, transgenic Arabidopsis plants that express W260 gene VI to two mRNA preparations derived from Col-0 control plants. The goal is to identify host genes whose expression is altered in response to a pathogenic form of gene VI. In the second experiment, we will compare two mRNA preparations from symptomless, transgenic Arabidopsis plants that expresses D4 gene VI to Col-0 control plants to identify host genes whose expression is altered by the presence of a nonpathogenic form of gene VI. for both experiments, we will utilize the hybrid experimental strategy, in which two biological and two technical replications are performed.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File