NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Description:Purpose: To identify genes whose expression is altered in cell cultures of Arabidopsis during hydrogen peroxide [H2O2] and/or nitric oxide [NO]-induced programmed cell death [PCD]. Rationale: H2O2 and NO are important signalling molecules that induce key cellular responses in plants. H2O2 generation following pathogen challenge is a ubiquitous process intimately linked with the Hypersensitive Response [HR], a form of PCD that represents a central player in an orchestra of defence responses. Plants also generate NO in response to pathogen challenge, and NO and H2O2 act together to initiate a barrage of defence responses. We have shown that Arabidopsis cultures represent an excellent system for biochemical and molecular dissection of those signalling and metabolic processes that lead to H2O2 and NO synthesis and culminate in defence gene expression and PCD. Arabidopsis cells rapidly produce H2O2 following elicitation, and H2O2 induces the expression of defence genes, PCD, and activation of MAP kinases. Bacterial challenge also elicits NO synthesis, NO alone stimulates PCD, and NO and H2O2 together result in even greater levels of PCD. Inhibition of gene expression, cGMP synthesis and caspase activity prevents PCD, but as yet there is a dearth of information relating to genes whose expression is modulated during PCD. Using mRNA differential display, we have already identified several such genes; it is timely now, therefore, to analyse the global patterns of gene expression during PCD by combining our well-defined culture system with the power of microarray technology. We propose to compare gene expression patterns between control cells and those exposed to H2O2 and NO. RNA will be extracted from cells after 3h, by which time cells are already committed to PCD but not yet dead. This will identify genes whose expression is modulated by H2O2 and/or NO; further work using northern analysis will differentiate genes induced by H2O2, NO or both. The identification of such genes will then facilitate functional investigation using knock-out technology and transient expression studies. Plant tissues: Suspension cultures Treatments: (i) Control-treated cells; (ii) H2O2 and NO-treated cells Genotype: Arabidopsis thaliana var. Landsberg erecta
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File