NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Description:Phytoremediation of soils contaminated with toxic levels of heavy metals requires the understanding of plant metal transport and tolerance. The numerous Arabidopsis thaliana studies have given scientists the potential for dissection and elucidation of plant micronutrient/heavy metal uptake and accumulation pathways. Thlaspi caerulescens, also a crucifer, tolerates and accumulates very high concentrations of Zn (40,000 ug/g in shoot) and Cd. Our studies of this heavy metal hyperaccumulation model plant have shown enhanced Zn transport into Thlaspi roots and shoots. We also showed altered regulation of ZNT1, a Zn/Cd transporter, contributing to the high Zn uptake. Isolation and characterization of Zn/Cd hyperaccumulation genes may allow expression in higher biomass plant species for efficient contaminated soil clean up. Our laboratory is taking several approaches, including microarray analysis in A. thaliana, to identify additional Zn transport, tolerance and nutrition-related genes whose homologues in T. caerulescens could be involved in heavy metal hyperaccumulation. Additionally, Zn-binding ligands involved in Zn homeostasis or tolerance may be identified. Previously, several A. thaliana Zn transporters were identified via yeast complementation. Because Zn requirement is very low, subtle differences in transporter mRNA levels in relation to plant Zn status may exist, and require extremely sensitive analysis as is found in microarray analysis. Gene products acting in concert to effect Zn uptake, which would not have been identified in complementation experiments, including multimeric transporter proteins, could also be identified. The proposed microarray comparison set consists of probes prepared from root RNA of A. thaliana (Columbia) seedlings hydroponically grown in complete nutrient medium (control) and Zn deficient seedlings grown in -Zn nutrient medium (experimental). Sequence analysis of identified A. thaliana genes will be used to investigate similarities to known Zn and other micronutrient transporters. Homologues of the A. thaliana genes from T. caerulescens and T. arvense, a related non-accumulator, will be isolated and analyzed for differential expression and a hyperaccumulation role.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File