NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Description:We isolated several genes from A. thaliana encoding proteins which share a novel zinc finger motif with LSD1, a negative regulator of cell death and defense response. One of these genes was designated LOL2. Due to an alternative splice site the LOL2gene encodes two different proteins, one of which contains an additional, putative DNA binding motif. Northern analysis demonstrated that LOL2transcripts containing the additional DNA binding motif are predominantly upregulated after treatment with both virulent and avirulent Pseudomonas syringae pv maculicola strains. Two lol2 T-DNA insertion alleles (genetic background: Ws-0) have been isolated, which, surprisingly, differentially regulate LOL2 transcription. One allele, lol2-2, is a reduction of function, while lol2-1 is an overexpression allele. In these lol2 mutants expression of known defense related genes like EDS1, NDR1, PR1, PDF1.2 and Nim1 is differentially regulated as well. Interestingly, one of the mutants also shows enhanced resistance to virulent and avirulent Peronospora parasitica isolates. The Northern data obtained for the lol2 mutants so far suggest that LOL2 acts in a signal transduction pathway leading to disease resistance. Induction of defense related genes appears to be negatively regulated by LOL2. Therefore, the lol2 mutants will prove to be a unique tool to further dissect the events leading to defense in plants. As LOL2 and other defense related genes are differentially expressed in the lol2 mutants, microarrays will be an ideal tool to further investigate the dependence of defense related genes on LOL2 expression and also to identify additional, unknown genes that are regulated by LOL2. For the microarrays, we propose to compare one lol2 mutant allele to the other. Alternatively, we could compare each lol2 allele to wild-type. We will consult with AFGC as to which experiment is most logical, although we prefer the former. We will use RNA isolated from sterile grown, 2-3 week old plants of the two lol2 mutants and/or the wild-type. If desired, plants can be grown in two batches per mutant/wild-type. RNA will then be isolated separately from the two batches and after quantification will be mixed for the microarray assay.
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