NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	26
Title:	Functional Genomics of Ozone Stress in Arabidopsis.
Date:	2005-01-26
Description:	Ozone is known to induce gene expression in plants. The roles of the induced genes and the molecular basis for the induction however largely remain to be elucidated. We will expose 2 week-old Arabidopsis seedlings to 200 ppb ozone for 1 h. RNA will be extracted from control and ozone-fumigated seedlings 3 h following the end of the fumigation period. The induction of the known anti-oxidant enzyme GST will be confirmed prior to submission of the RNA samples to GARNet. Changes in gene expression will be assessed by hybridising microarrays with fluorescently labelled cDNA prepared from control and ozone-fumigated seedlings. The role of selected genes will be inferred from their sequence and further established by over expression under the control of the 35S or cell-specific promoters and by searching for mutants among single transposon or T-DNA insertion collections. Plants will be analysed on the basis of traits that are known to be affected by ozone including growth, flowering and stomatal responses. In addition, the ozone calcium signature we have reported previously (Clayton et al.1999) will be studied by crossing transgenic or mutant plants with apoaequorin or yellow cameleon 2.1-transformed plants.
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Tissue	Stock Code	Cel File
Short_1-1_ozone_Rep1_ATH1	194	Whole Plant	N1093	ES001_ATH1_A1-mcain-ozo.CEL
Short_1-1_ozone_Rep1_ATH1	Treatment: Fumigation with 500ppb Ozone for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The ozone was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and extracted immediately after fumigation.
Short_1-2_control_Rep1_ATH1	196	Whole Plant	N1093	ES001_ATH1_A2-mcain-con.CEL
Short_1-2_control_Rep1_ATH1	Treatment: Fumigation with scrubbed air (filtered through charcoal and purafill) for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The air was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and RNA extracted immediately after fumigation.
Short_1-3_ozone_Rep2_ATH1	1570	Whole Plant	N1093	Short_1-3_ozone_REP2_ATH1.CEL
Short_1-3_ozone_Rep2_ATH1	Treatment: Fumigation with 500ppb Ozone for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The ozone was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and extracted immediately after fumigation.
Short_1-4_control_Rep2_ATH1	1572	Whole Plant	N1093	Short_1-4_control_REP2_ATH1.CEL
Short_1-4_control_Rep2_ATH1	Treatment: Fumigation with scrubbed air (filtered through charcoal and purafill) for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The air was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and RNA extracted immediately after fumigation.
Short_1-5_ozone_Rep3_ATH1	1571	Whole Plant	N1093	Short_1-5_ozone_REP3_ATH1.CEL
Short_1-5_ozone_Rep3_ATH1	Treatment: Fumigation with 500ppb Ozone for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The ozone was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and extracted immediately after fumigation.
Short_1-6_control_Rep3_ATH1	1573	Whole Plant	N1093	Short_1-6_control_REP3_ATH1.CEL
Short_1-6_control_Rep3_ATH1	Treatment: Fumigation with scrubbed air (filtered through charcoal and purafill) for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The air was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and RNA extracted immediately after fumigation.