| Description: | We are interested in identifying gene expression changes that occur during shoot development in Arabidopsis. We have set up a system to regenerate shoots from root explants employing a procedure originally developed by Valvekens to generate transgenic Arabidopsis plants. We have improved on the procedure by finding Arabidopsis lines that develop shoots synchronously and in great profusion. We are using this system in conjunction with mutants to study the hormonal control of organogenesis. We expect to see substantial gene expression changes during shoot development, as many differences as one might expect to find in comparing roots to shoots. In the regeneration procedure, we first incubate root explants on a callus induction medium (CIM) during which time they acquire 'competence' to respond to hormones that induce shoot formation. Explants are then transferred to cytokinin-rich shoot induction medium (SIM) where they organize meristems and undergo shoot morphogenesis. We are interested in identifying a) genes expressed during the acquisition of competence that may encode components of the hormone signaling pathway and b) genes expressed during shoot formation that may be involved in organizing meristems and organ primordia. A typical time course is 18 days total, 4 days on CIM and 14 days on SIM until shoots begin to emerge. Since this process occurs in phases and stretches out over a fairly long time course, we would like to request special permission to survey the microarrays with probes representing 3 time points: zero time derived from freshly explanted roots, a pooled 'early' set representing 2 and 4 days on CIM and 2 and 4 days on SIM, a pooled 'late' set representing 6, 8, 10 and 12 days on SIM. The pooled time points will be used to generate our own smaller arrays for more extensive time course studies and analysis of mutants with defects in shoot organogenesis. |
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.