NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Description:Purpose: We have isolated several Arabidopsis mutants which are defective in protein import into chloroplasts. The purpose of our experiments, using the microarrays, is to identify and characterize genes that are differentially expressed between the wild-type and mutants. Knowledge obtained from this study will not only help us understand the mechanism and regulation of chloroplast protein import but will also further our understanding of chloroplast biogenesis in general. Rationale: Using a transgene-based screening strategy similar to the one used to isolate yeast mitochondria protein import mutants (1), my laboratory has isolated several Arabidopsis mutants that are defective in protein import into chloroplasts. The traditional way of characterizing these mutants is to identify the defective loci by chromosome walking and then characterize the gene products by homology search, localization and identification of interacting components. All of these approaches are labor-intensive and do not always warrant a meaningful return. In contrast, the most comprehensive approach of unveiling the physiological consequences caused by the mutation, i.e. understanding the complete function of the gene product, is the one offered by the microarray technique. By hybridizing the microarrays using probes made from the mutants and the wild type, the entire spectrum of genes that are differentially expressed among the mutants and the wild type can be identified. In addition, for mutations that cause a severe reduction in the mRNA level of the encoding loci, microarrays also offer the possible advantages of identifying the mutant genes without going through the difficult and labor-intensive work of chromosome walking. However, in this proposal, we will first use a mutant for which the mutation locus has been identified and compare its gene expression pattern with its corresponding wild type. The mutant has a pale green phenotype early in development and in young tissues. The leaf color eventually darkens to a level comparable to the wild type. Plant tissue and treatment Whole seedlings grown on synthetic medium (1X MS salts and 2% sucrose) under 16 h light/8 h dark cycle at 22 degree C for 7 days. genotypes for RNA preparation Columbia Reference: 1. Maarse et al., EMBO (1992) 11, 3619-3628.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File