| Description: | There are about 1000 protein serine/threonine kinase genes identified in Arabidopsis, out of which, approximately 600 encode for RLKs (receptor like kinases). The functions of majority RLKs are largely unknown. The Arabidopsis cell wall associated kinase (WAK) gene family contains five highly related members whose products are suited for exchanging signals between the cell wall and the cytoplasmic compartment. We have recently shown that the dexamethasone (dex)-inducible WAK4 antisense expression can effectively control WAK protein levels. WAK suppression results in cell elongation inhibition and morphological alternations (Lally et al., 2001 Plant Cell, in press). We are very interested in how WAKs are specifically involved in cell expansion and Arabidopsis development. Our analyses have shown that the induced WAK suppression causes a substantial decrease of expansin transcript suggesting that WAK suppression may interact with or influence expansin. We hypothesize that there are other genes, particularly those involved in cell expansion and development, could also be affected by WAK suppression. In this proposed study, we would like to systematically compare the gene expression profile of plants in which the WAKs are suppressed by dex with that of plants of normal WAK expression (no dex treatment). Two identical groups of WAK4 antisense plants will be grown under standard conditions. At 8-leaf stage one group will be sprayed with 20 micro molar dex and the other group will be sprayed with a mock solution. 48 hours after spraying leaf tissue will be harvested from both group and used for RNA extraction. This is when phenotypes are starting to develop. We plan to request duplicate slides for each treatment. The microarray analysis of the isolated RNA will help us identify genes that are regulated in response to WAK suppression. The elucidation of these genes would provide valuable insight as to how WAKs function in cell expansion, and perhaps help us identify WAK-mediated signaling pathway components. |
NASCArrays Information at The BAR
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