| Description: | Sulfur is used to make diverse plant constituents among them the 23 different sulfur containing glucosinolates believed to be important in plant defense. We are interested in the link between sulfur nutrition, plant defense and plant growth. In earlier experiments we have looked at the influence of sulfate and sulfate starvation on activity and expression of specific proteins and sulfur containing compounds in cruciferous plants including Arabidopsis. In collaboration with Shauna Somerville we have used a small DNA microarray to analyse the effect of sulfate starvation on the transcript profile. These studies indicate that some groups of transcripts and proteins are influenced by sulfate nutrition including e.g lectin like jasmonate inducible proteins and thioglucosidases. Many potential important genes in the metabolic pathways of interest are, however, missing on this smaller array. We therefore want to use a more complete DNA microarray for a better profiling. We will use root tissue of Arabidopsis thaliana ecotype Col. from 3-4 week old plants. Sample plants will be grown on sulfate free medium where the sulfate salts have been replaced by cloride salts, while the control will be grown on medium with sulfate containing salts. At 3 weeks stage (stage 0), plants will be given a complete sulfate containg medium and roots harvested 6, 12 and 24 h thereafter to analyse transcripts regulated during recovery We would like to use 3 biological replicates at each timepoint and therefore ask for 12 arrays. If fewer arrays are available we will give priority to the stage 0 and 12 h after feeding. We are also open to use fewer time points and more replicates if this is recommended. |
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
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