NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Description:Senescence of higher plant is the final phase of plant organ.s development, involving the degradation of cellular constituents whose amino acids and sugars are transported for use in other organs. Vacuolar remodeling is a key feature of senescence in which the vacuole is converted from multifunctional organelles to a lytic organelle that mediates extensive autophagy. An emerging new aspect of vacuolar remodeling and senescence is the role of ER-derived protease precursor vesicles in transforming the vacuole. The protease precursor vesicles contain a unique member of the cysteine protease family that terminates in the ER-retention sequence KDEL. There are two expressed KDEL-protease genes in Arabidopsis that we have analyzed with RT-PCR. Vacuolar remodeling is an essential feature of plant development and in stress reaction occurring whenever there are large changes in tissue and cellular morphology. A knockout of KDEL-protease genes could impede vacuolar remodeling and the formation of ER-derived vesicles. We screened the Univ. Wisconsin T-DNA insertion pool with RT-PCR using primers designed to be gene specific for the two Arabidopsis KDEL-protease genes and isolated an insertion mutant for one of the genes with the insert in the first exon. The lack of the KDEL protease results in a phenotype where leaf senescence is greatly delayed with the mutant plants leaves remaining green long after the wild type plant.s leaves have died. This mutant also appears to exhibit increased drought tolerance. A complete analysis of the gene expression of this mutant using array technology will provide us with means to assess whether the mutant leaves persist in a normal vegetative state, or whether the leaves that persist because senescence gene products are not effectively utilized. We propose to use the Arabidopsis arrays to study the gene expression of the KDEL-protease knockout in comparison with the wild type plant. We will compare the gene expression of wild type green leaves (1 slide), wild type early senescence (1 slide), and mutant persistent green leaves (2 slides for replicate). These experiments will determine the whether the mutant leaves persist in a normal vegetative state or whether the mutant leaves express senescence specific genes but in the absence of vacuolar remodeling mediated by the ER-derived protease precursor vesicles the leaves retain a vegetative phenotype.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File