Description: | Our final goal of this project is to identify the factors that confer the ability to cultured cells for the production of secondary metabolites. A few of the cultured cell lines have been reported to maintain the ability to produce secondary metabolites,however, it is well known that most of the cultured plant cells do not display the ability to produce secondary metabolites which are produced in plants. We are interested in analyzing why most of the cultured cells do not produce the secondary metabolites that are produced in the plant tissue and why some cultured cell lines can maintain the ability to produce secondary metabolites. Although it is most likely that the genes which are involved in the production of secondary metabolites are inactive in most of cultured cells, it is not characterized which genes are expressed between differentiated tissue cells and dedifferentiated cultured cells or between the cultured cell lines that produce a certain metabolites. To approach the key factors that involved in the production of secondary metabolites in cultured cells, as the first step, we will compare the gene expression pattern between leaves, roots and cultured cells by isolating mRNAs form Arabidopsis plants and cultured cells, and characterize the difference between cultured cells and intact plants on the gene expression level |