| Description: | Jasmonic acid, synthesized in octadecanoid pathway is known to be a signal trasduction molecule involved in regulation of different cellular processes in plants. The role of methyl jasmonate in plant fertility was established by characterization of Arabidopsis male sterile mutants fad3-2 fad7-2 fad8 and opr3. The former mutant lacks hexadecatrienoic acids (16:3) and linolenic (18:3) fatty acid precursor of jasmonate while the latter lacks 12-oxophytodienoic acid reductase, one of the enzymes of jasmonate synthesis. Thus these mutants are unable to synthesize jasmonic acid. These male sterile mutants are made fertile by spraying with methyl jasmonate on flower buds especially during late stages of flower development. The Arabidopsis coi1 mutant, which is unresponsive to jasmonate and related compounds, is also male sterile but fertility is not restored by jasmonate treatment. Therefore it is suggested that jasmonate activates the set of genes during the late stages of anther development and which are probably involved in pollen maturation and fertility. The purpose of this experiment is to identify the jasmonate regulated genes in anther and subsequently assign their functional role in male fertility in Arabidopsis. The male sterile mutant mutant opr3 (ecotype Wassilewskija) will be grown in standard environmental conditions and will be used as a control to isolate a specific stage of anthers. Other plants will be sprayed with jasmonate and anthers will be isolated after 1 hr of the treatment. The RNA from both control and jasmonate treated anthers will be used to make probes for hybridization to microarrays. Ref: 1. Stintzi, A. & Browse, J. (2000) Proc. Natl. Acad. Sci. USA 97, 10625-10630. 2. McConn, M. & Browse, J.(1996) Plant cell 8, 403-416. 3. Feys, B. J. F., Benedetti, C. E., Penfold, C. N. & Turner J. G. (1994) Plant cell 6, 751-759. |
NASCArrays Information at The BAR
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