NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Description:RNA viruses such as tobacco mosaic virus (TMV) have evolved specific interactions with their host's cellular machinery that facilitate virus replication, cell-to-cell movement and systemic spread. Ultimately, these interactions are also responsible for rendering the plant either resistant or susceptible to virus invasion. The objective of this proposal is to assess the regulation of host genes upon the establishment of a systemic virus infection that results in the display of disease symptoms. This study is an extension of an earlier microarray experiment (Proposal # 945-CJC-890-HFG-1) involving the identification of genes induced in response to TMV infection in inoculated leaf tissues. For these studies, the Arabidopsis ecotype Shahdara was chosen because it supports the rapid systemic movement of TMV throughout the plant and exhibits severe disease symptoms, such as leaf curling, stunting, late bolting and necrosis within 14 days of virus inoculation. These responses are similar to those seen in other TMV infected plant species including tobacco, tomato and pepper. Thus, the TMV-Shahdara system is ideally suited for the study of plant-virus interactions. For the current set of experiments, mature rosette leaves of Shahdara plants will be inoculated with 10ug of TMV. At 14 days post inoculation, total RNA will be extracted in duplicate sets from uninoculated upper leaves of TMV infected Shahdara plants. Previous experiments have shown that TMV moves into the uninoculated upper leaves of Shahdara within 5 - 7 days of inoculation and reaches a maximum level of accumulation within 14 days post inoculation. Control RNA will be extracted from upper leaves of mock inoculated Shahdara plants. Control and test poly A+ RNA extracted from these total RNA samples will be submitted for microarray analysis to identify the genes induced at this infection stage. The gene expression profile obtained from this microarray analysis will be useful in identifying plant genes that are altered in tissues that display virus induced symptoms. Such genes should reflect the physiological changes imparted by the virus and provide insight into the disease process. Results from this experiment can also be compared to results from our previous experiment to assess the changing status of specific host genes at different stages of infection. Together this information should provide new insights into how plants respond to the establishment of a virulent virus infection.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File