| Description: | One of the most dramatic effects of auxin is its ability to trigger the formation of lateral roots. In this manner auxin is of crucial importance for the development of the root system of plants. Nevertheless, differential screenings to identify genes that are expressed in relation to lateral root formation are scarce. For that reason we have recently started to investigate auxin-induced genes in arabidopsis roots (Neuteboom et al., Plant Mol Biol 39: 273-287, 1999; Neuteboom et al., DNA Research 6: 13-19, 1999). Despite the simplicity of our screening method, our studies showed that interesting genes with novel expression patterns can easily be found in roots. To our opinion it will be very rewarding to investigate auxin-induced changes in gene expression in roots in relation with lateral root formation with the microarray technique. To make the screening more useful for the wider scientific community interested in fundamental plant biology, thus avoiding any special tissue culturing methods, we propose the following: Seeds of the wild-type Columbia ecotype will be sown in lines on plates with half strength MS medium (containing 10 gram per liter sucrose) covered with a fine mesh nylon cloth which allows easy transfer of seedlings. The plates will be incubated at an angle of 60 degrees in a growth chamber set at 21 C under a 16 h illumination cycle. Ten days after germination the seedlings, which on average will have a 2 cm long root, will be transferred to fresh plates (control or containing 1 micromolar of the auxin 1-NAA). After 24 h the roots of the seedlings will be harvested and RNA will be isolated. We propose to pool the roots from at least 10 plates for both treatments and this in duplicate, thus resulting in two 'control RNA' samples and two 'NAA RNA' samples. Differential signals after the use of probes derived from control RNA and NAA mRNA are of interest for: A: Those interested in genes expressed in developing lateral root primordia since after 24 hours of NAA treatment almost all stages of lateral root formation can be found at much higher frequency than in the controls. B: Those interested in gene expression as a consequence of lateral root formation, which is a novel aspect of development of the root system. C: Those interested to find novel auxin-inducible genes. |
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.