| Description: | The zinc finger trascription factor DAG1 controls seed dormancy, in that homozygous dag1 T-DNA knock-out mutant seeds are non-dormant and do not need a red light pulse to germinate (Papi et al., Genes & Development, 14, 28-33 (2000)). The gene DAG1 is expressed in the phloem of developing siliques but not in the embryo, and this represents a first example of maternal control of seed germination, excluding mutants of the seed coat. The phytochrome?mediated pathway for seed germination is still required in dag1 mutant seeds, since a far-red light pulse inhibits dark germination of the mutant seeds. Thus DAG1 may ensure the inactivity, for dormancy initiation, of a component - transported from the mother plant to the seed - that would be activated, to trigger germination, as a consequence of red light perception. Purpose of the microarray screening will be to identify genes that are deregulated in the dag1 mutant as compared to the Wassilewskija wild type, with the scope of identifying the regulatory target(s) of the DAG1 transcription factor. This will allow to shed considerable light on the mechanism of red light?induced seed germination in Arabidopsis. Microarrays should be analyzed with RNA-derived probes from developing siliques - stages 12 to 16 - of dag1 plants and from equivalent tissues of Wassilewskija wild type plants. |
NASCArrays Information at The BAR
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