NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Description:The Dof proteins are transcription factors widely distributed only within the plant kingdom, characterized by the Dof domain, a C2-X21-C2 zinc finger. The unusual conservation of this domain coupled with the presence of the Dof transcription factors in all plants, suggests a crucial role of these proteins in regulating different functions typical of and of universal relevance in plants. By means of a reverse genetic approach, we demonstrated that the Arabidopsis Dof protein DAG1 is involved in the control of red light-dependent seed germination (Papi et al., Genes & Development, 14, 28-33 (2000)). We subsequently identified a T-DNA insertion line in another uncharacterized gene of the Arabidopsis Dof family. A mutant line was identified and the Dof gene was denominated DAG2 for its sequence similarity with DAG1 - 57% aminoacidic identity on the whole protein. dag2 was shown to be a knock-about mutant, as the N-terminus of DAG2 is expressed in translational fusion with the GUS reporter gene. DAG2 and DAG1 might be involved in interacting pathways or even regulate with opposite roles the same gene(s) based on their overlapping expression patterns and on the opposite phenotype of the respective mutants. Purpose of the microarray screening will be to identify genes that are deregulated in the dag2 mutant as compared to the Wassilewskija wild type, with the scope of identifying the regulatory target(s) of the DAG2 transcription factor. This will allow to establish whether DAG2 and DAG1 have opposite effects in controlling the same regulatory circuit(s) in Arabidopsis. According to the DAG2 and DAG1 expression patterns, microarrays should be analyzed with RNA-derived probes from developing siliques - stages 12 to 16 - of dag2 mutant plants and from equivalent tissues of Wassilewskija wild type plants. The analysis concerning DAG2 could be coordinated with that of DAG1, just submitted from our laboratory - proposal number 957-JGE-935-JEJ.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File