| Description: | The ongoing rise in atmospheric CO2 concentration is likely to be the most significant and pervasive environmental factor affecting plants in the 21st century. A doubling of atmospheric CO2 will impact all plants, regardless of ecosystem or agricultural importance. Furthermore, because of the fundamental nature of CO2 in plant metabolism, it is likely that elevated CO2 will affect a very large number of metabolic pathways. Work in our lab and others has detailed many of the specific effects of elevated CO2 on the biochemistry and molecular biology of photosynthesis [for review see Moore, Cheng, Sims, & Seemann {1999} Plant Cell and Environment 22: 567-582] and it is clear that there are similar dramatic effects on many other plant processes. At the level of gene expression, we and others have begun to document the effects of elevated CO2 on the expression of a few genes in Arabidopsis, but it is likely that the number of genes whose expression is affected is exceptionally large. Microarray technology offers the ideal approach for obtaining a global overview of the effects of elevated CO2 on plant gene expression. This technology will enable us to examine the effects of elevated CO2 on the expression patterns of genes involved in a variety of plant metabolic pathways including carbon fixation, light reactions, photorespiration, respiration, glycolysis and nitrogen metabolism, as well as the associated signaling pathways. Arabidopsis [ecotype Columbia] will be grown hydroponically at optimal nutrient concentrations at either 1000 microl/L or 360 microl/L [ambient] CO2. Vegetative tissues [roots and shoots] will be harvested for RNA preparation at maturity prior to the transition to flowering. These initial experiments will allow us to isolate the effects of elevated CO2 on gene expression and minimize any effects due to developmental changes and nutritional deficiencies. Results from this experiment will identify coordinate patterns of transcriptional regulation. We will use this information to focus our future research on subsets of regulated genes. Examination of CO2 effects on the transcription of these genes will also include developmental time courses, varied nutrient regimes, preindustrial CO2 concentration, and the use of different mutant backgrounds. These studies will help unravel the complex responses of plants that will occur in the doubled atmospheric CO2 content of the 21st century. |
NASCArrays Information at The BAR
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