NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 20 |
| Title: | The transcriptome of A.thaliana during UV-induced Programmed Cell Death |
| Date: | 2004-06-23 |
| Description: | We have published results demonstrating that UV-C induces apoptotic-like changes in Arabidopsis (Danon and Gallois FEBS lett (1998) 437: 131-136). The Programmed Cell Death "phenotype" of nucleus shape, DNA laddering caspase-like activity is similar to what has been described in other plant PCD. This demonstrates that UV-C is an appropriate and controllable trigger to study PCD in plants. Using selected mutants and a set of chosen conditions we are aiming at identifying genes that are part of the PCD pathways in plants and filter out the general stress response. We are asking for a medium size experiment knowing that additional microarray analysis are likely to be required to refine the results obtained. We are currently awaiting the results of Affymetrix RNAs hybridisation of PCD-induced wild type that will define the appropriate single time point of the proposed analysis. The rationale is to vary treatments in order to distinguish changes in gene transcription arising from general cellular stress responses to the trigger used from those specific to PCD: 1. We will use wild-type Arabidopsis seedlings as a negative control to provide the basal gene expression pattern. 2. A dose of UV-C radiation of 1KJ/m2. We will irradiate wild type with this non PCD-inducing dose of UV-C radiation to identify sets of genes that respond to UV-induced damage but whose expression is not linked to cell death. 3. A dose of UV-C radiation of 50 KJ/m2. We will irradiate wild type with a PCD-inducing dose of UV-C radiation to identify sets of genes that respond to UV-induced damage and cell death.4. We have shown that the cell death induced by UV is light dependant, the control, sub-inducing dose and inducing dose treatments will be repeated and the plants kept in the dark until RNA sampling. In those conditions there is no PCD. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Gallois_1-10_UV-1kJ_Rep1_AG | 146 | aerial part of seedling | PG001_AG_PG10.CEL | ||
| Treatment: Sample sublethal dose 1 kJ/m2-Dark. Seedlings incubated in the dark after UV treatment and harvested 30 min after UV-C radiation UV-C treatment as described in Danon and Gallois (1998) FEBS Letters, 437, 131-136 | |||||
| Gallois_1-11_UV-50kJ_Rep1_AG | 148 | aerial part of seedling | PG001_AG_PG11.CEL | ||
| Treatment: Sample lethal dose 50 kJ/m2-Dark. Seedlings incubated in the dark after UV treatment and harvested 30 min after UV-C radiation UV-C treatment as described in Danon and Gallois (1998) FEBS Letters, 437, 131-136 | |||||
| Gallois_1-15_control_Rep2_AG | 150 | seedling protoplasts | PG002_AG_PG15.CEL | ||
| Gallois_1-16_UV-1kJ_Rep2_AG | 152 | aerial part of seedling | PG002_AG_PG16.CEL | ||
| Treatment: Sample sublethal dose 1 kJ/m2-Dark. Seedlings incubated in the dark after UV treatment and harvested 30 min after UV-C radiation UV-C treatment as described in Danon and Gallois (1998) FEBS Letters, 437, 131-136 | |||||
| Gallois_1-17_UV-50kJ_Rep2_AG | 804 | aerial part of seedling | PG002_AG_PG17.CEL | ||
| Treatment: Sample lethal dose 50 kJ/m2-Dark. Seedlings incubated in the dark after UV treatment and harvested 30 min after UV-C radiation UV-C treatment as described in Danon and Gallois (1998) FEBS Letters, 437, 131-136 | |||||
| Gallois_1-9_control_Rep1_AG | 144 | seedling protoplasts | PG001_AG_PG9.CEL | ||