| Description: | The microarray analysis will be used to help identify gene regulation that leads to temperature-sensitive arrest of the broccoli meristem at the transition to floral initiation. Broccoli normally develops a ramified inflorescence, without flower bud development. After a certain period, meristems begin to make flower buds instead of more inflorescences. The meristem is arrested at this transition if the temperature is too high. This arrest is a good model for studying the regulation of inflorescence development, and is also a substantial agricultural problem. Broccoli is a good model system for studying the stages of reproductive development in crucifers because the stages exaggerated in broccoli over Arabidopsis. The homology is high enough between these species that reasonable hybridization can be expected. There are many genes that are candidates for being involved in the developmental arrest. These genes include CAL, LFY, AP1, TFL, AG, CBF1. Some have been identified as associated with the similar arrest that occurs in cauliflower. The genes actually in the regulatory sequence will be identified by high differential expression in the microarrays. This analysis will be particularly valuable in identifying additional genes that are not currently suspected. Using these results, and what is currently known about the mutual regulation among the MADS homeotic, we will propose a model for heat regulation of inflorescence genes. This model would provide the working hypothesis for an ongoing investigation using mapping and in situ hybridization to study regulation of the arrest. The RNA will be isolated from the inflorescence meristem of broccoli at the stage of development most responsive to high temperature. Control plants will be held at 22/15C (D/N) for three days before isolation, treated plants at 35/22. The genotype is a highly susceptible broccoli (Brassica oleracea var. italica) F1 hybrid, PS19590, in which we are mapping heat-tolerance QTLs |
NASCArrays Information at The BAR
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