NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:198
Title:ATP CITRATE LYASE
Date:2005-03-11
Description:Cast as a central molecule in plant metabolism, acetyl-CoA plays a major role in many interconnected primary and secondary biochemical pathways, producing classes of compounds such as fatty acids, waxes, flavonoids and isoprenoids. Since acetyl-CoA is membrane-impermeable, synthesis is thought to occur in the subcellular compartment where it is required. Several acetyl-CoA-generating mechanisms have been proposed including one involving ATP citrate lyase (ACL). ACL catalyzes the ATP-dependent reaction between citrate and CoA to form oxaloacetate and acetyl-CoA. Our molecular characterizations of Arabidopsis ACL cDNAs indicate that the plant enzyme consists of two dissimilar subunits (A and B). Using subunit-specific antibodies and activity assays, we have shown that ACL is located in the cytosol, and is not detectable in plastids, mitochondria, or peroxisomes. ACL mRNA a accumulates preferentially in cells requiring cytosolic acetyl-CoA. Antisense ACL-A plants with reduced ACL activity have a distinct phenotype including: miniaturized organs, small cell size, aberrant plastid morphology and reduced cuticular wax. Our initial characterizations indicate that ACL may be providing acetyl-CoA for cytosolic acetyl-CoA-derived phytochemicals. We wish to compare mRNA accumulation profiles in elongating inflorescence stems of antisense ACL and wild type Arabidopsis thaliana (Columbia). Inflorescence stems have been selected for analysis in that the ACL mutants have easily comparable developmental stages, and adequate mass for the extraction of RNA and further biochemical studies. We will use the wealth of resulting data, along with other experimental data to formulate testable hypotheses to address several questions. (1) How are genes involved in cytosolic acetyl-CoA-requiring pathways effected? (2) Are genes of other acetyl-CoA generating mechanisms effected (i.e., Do alternative acetyl-CoA-generating pathways compensate for the lack of cytosolic acetyl-CoA? (3) What other metabolic pathways are involved with or linked to ACL? This analysis may also lead to the discovery of currently unknown pathways requiring ACL-derived acetyl-CoA. The use of microarray analysis would greatly enhance our current analysis of antisense ACL plants and thus our investigation into the role of ACL in acetyl-CoA generation.
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