NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:197
Title:MUTANTS RESPONSE TO IAA
Date:2005-03-11
Description:BACKGROUND: Individual members of Auxin Response Factor (ARF) transcriptional regulators may control specific types of auxin responses. Our mutant/overexpression analysis supports this notion. Mutations in MP (IAA24/ARF5) and ARF7 (NPH4/MSG1) confer highly specific (!) auxin insensitivity. Phenotypes implicate MP and ARF7 in partially non-overlapping aspects of auxin-dependent cell elongation, but only MP in vascular differentiation, suggesting regulation of two sets of partially non-overlapping genes. RATIONALE: So far, we have identified >20 genes with highly specific auxin inducibility which is dependent on the activity of MP and/or ARF7. This is evidenced by both reduced auxin inducibility in mp and msg1 mutants and enhanced inducibility in corresponding MP and ARF7 overexpression plants. Inducibility of three of these genes is selectively affected only by MP gene activity and all three of these genes are expressed specifically in vascular tissues. Thus, large-scale differential auxin inducibility profiles could identify most auxin inducible genes in the developmental pathway of each ARF gene; e.g. for MP in vascular development. Comparison of mp versus Col-0 tissue would additionally provide a huge selective collection of vascular genes, since otherwise normal mp cotyledons nearly devoid of vascular tissues can be obtained. TREATMENT/GENOTYPE: We suggest to assess rapid (<20min) inducibility at 20µM versus 0µM IAA in Col-0 (1), in mp (2) and in msg1 (3) mutants (both are in Col-0). PRIORITIES/LINKS: We would suggest the sequence (1), (2), (3) as described above. This would involve 6 or 12 hybridizations, but likely (1) and possibly (3) will be suggested by others, and one should then coordinate the induction/tissue conditions towards an integrated experiment. Alternatively, we could supply RNA for all three experiments as microarray facilities are becoming available in successive rounds. TISSUE/RNA: RNA from apical seedling organs will be prepared by a senior post-doc, who has developed a highly reproducible induction protocol for immersed Col-0 seedlings. We have >20 genes with known profiles to test each RNA sample. These internal controls may also help in further microarray optimization. SIGNIFICANCE:(1) is of obvious general interest to entire auxin community. Among other goals, (2)(3) would provide the first comprehensive collection of Arabidopsis vascular genes (high general interest; later spec. microarray). PERSPECTIVE: Our long-term goal is understanding signaling in vascular development and we would concentrate on the class of specifically MP dependent gene expressions, that would be further characterized by expression pattern and reverse genetic phenotype.DOCUMENTATION: We can provide quantified Northern data to demonstrate the feasibility of the approach. RELEASE: (1)(3) immediately, (2) 3 months LIT: EMBO J 17, 1405-1411 Plant Phys 115, 419-426 Plant Phys 118, 1265.1275
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