NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:19
Title:Profile expression of genes involved in plant reaction to lead:uptake tolerance and defense.
Date:2003-06-08
Description:The aim of the experiment is to study the pattern of genes expression involved in defense to heavy metals and their transport and distribution within plant organs. Most of the plant species inhibits heavy metals entrance to the roots and their transport and accumulation to/in above ground organs after toxic ions penetrate roots. Some species of Brassicacea differ in the pattern of defense gene expression in the roots in a way that allow for greater transport of toxic ions to the above ground part. If so, by harvesting such plants toxic elements will be extracted from the soil. Identification of these differences in the gene expression is crucial in development of the newly emerging environmental biotechnology phytoremediation in which plants as well as being green lungs play also a role of a green liver. Results of our studies with mustard plants showed among others that exposure for 12 hr to 12.5mg of Pb/1L of hydroponics solution is efficient to increase of constitutive genes expression and in de novo induction of genes expression coding for Pb tolerance and that this response is organ specific. Sequencing of the whole genome of A. thaliana opens the opportunity to study this phenomenon at molecular level more widely including the signaling (two-component system) and plasmolemma and tonoplast transporters genes. Therefore in our further steps we are going to use arabidopsis as a model plant and the transcriptomics aproach is the only way for such studies. At first based on results with mustard physiological study we will conduct for elucidating arabidopsis plant responses to lead in order to choose the most appropriate dose and time of treatment. In the trascriptome experiment we would like to study the profile gene expression in above ground parts and in the roots of arabidopsis plants in response to toxic ions of lead. Results of this study besides of promissing applicable aspects will also lead to better understanding plant acclimation and adaptation to antropogenic stresses. We expect to obtain mutant(s) tolerant to Pb and if sothey will be provided to NASC.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Gawronski_1ASWGcL136rosette leaves N933SG001_AG_1ASWGcL.CEL
Gawronski_2ASWGcR138roots N933SG001_AG_2ASWGcR.CEL
Gawronski_3ASWG25PbL140rosette leaves N933SG001_AG_3ASWG25PbL.CEL
Treatment: between developmental stages 3.90 and 5.10 (complete rosette growth and first flower bud visible) along with nutrients changes - 25 ppm of Pb as Pb(NO3)2 was added to the nutrient solution twice (in week interval)
Gawronski_4ASWG25PbR142roots N933SG001_AG_4ASWG25PbR.CEL
Treatment: between developmental stages 3.90 and 5.10 (complete rosette growth and first flower bud visible) along with nutrients changes - 25 ppm of Pb as Pb(NO3)2 was added to the nutrient solution twice (in week interval)
Gawronski_5ASWG50PbL137rosette leaves N933SG001_AG_5ASWG50PbL.CEL
Treatment: between developmental stages 3.90 and 5.10 (complete rosette growth and first flower bud visible) along with nutrients changes - 50 ppm of Pb as Pb(NO3)2 was added to the nutrient solution twice (in week interval)
Gawronski_6ASWG50PbR139rosette leaves N933SG001_AG_6ASWG50PbR.CEL
Treatment: between developmental stages 3.90 and 5.10 (complete rosette growth and first flower bud visible) along with nutrients changes - 50 ppm of Pb as Pb(NO3)2 was added to the nutrient solution twice (in week interval)