NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	167
Title:	AtGenExpress:Pathogen Series:Response to Botrytis cinerea infection
Date:	2005-02-02
Description:	AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate.Responses to B. cinerea infection were assayed in wild-type Col-0 plants at 18 and 48 hours after inoculation of adult leaves. Leaves were inoculated by placing 4 5-ul drops of a 5x10e5 spore solution on each leaf. Control leaves were spotted with droplets of 24g L-1 potato dextrose broth medium.
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Tissue	Stock Code	Cel File
BC181-1	2709	leaves	N6673	BC1811.cel
BC181-1	Treatment: Conidiospores of Botrytis cinerea were collected with sterile water from 2-week-old plates, pelletted and resuspended in 24 g L-1 sterile potato dextrose broth (Difco, Detroit, USA). Conidiospores were diluted to 5X10^5 spores/ml and pre-germinated at RT for 3 hours. Four 5 ul droplets were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 18 hpi.
BC181-2	2710	leaves	N6673	BC1812.cel
BC181-2	Treatment: Conidiospores of Botrytis cinerea were collected with sterile water from 2-week-old plates, pelletted and resuspended in 24 g L-1 sterile potato dextrose broth (Difco, Detroit, USA). Conidiospores were diluted to 5X10^5 spores/ml and pre-germinated at RT for 3 hours. Four 5 ul droplets were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 18 hpi.
BC182-1	2711	leaves	N6673	BC1821.cel
BC182-1	Treatment: Conidiospores of Botrytis cinerea were collected with sterile water from 2-week-old plates, pelletted and resuspended in 24 g L-1 sterile potato dextrose broth (Difco, Detroit, USA). Conidiospores were diluted to 5X10^5 spores/ml and pre-germinated at RT for 3 hours. Four 5 ul droplets were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 18 hpi.
BC481-1	2712	leaves	N6673	BC4811.cel
BC481-1	Treatment: Conidiospores of Botrytis cinerea were collected with sterile water from 2-week-old plates, pelletted and resuspended in 24 g L-1 sterile potato dextrose broth (Difco, Detroit, USA). Conidiospores were diluted to 5X10^5 spores/ml and pre-germinated at RT for 3 hours. Four 5 ul droplets were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 48 hpi.
BC482-1	2713	leaves	N6673	BC4821.cel
BC482-1	Treatment: Conidiospores of Botrytis cinerea were collected with sterile water from 2-week-old plates, pelletted and resuspended in 24 g L-1 sterile potato dextrose broth (Difco, Detroit, USA). Conidiospores were diluted to 5X10^5 spores/ml and pre-germinated at RT for 3 hours. Four 5 ul droplets were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 48 hpi.
BC482-2	2714	leaves	N6673	BC4822.cel
BC482-2	Treatment: Conidiospores of Botrytis cinerea were collected with sterile water from 2-week-old plates, pelletted and resuspended in 24 g L-1 sterile potato dextrose broth (Difco, Detroit, USA). Conidiospores were diluted to 5X10^5 spores/ml and pre-germinated at RT for 3 hours. Four 5 ul droplets were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 48 hpi.
CT181-1	2715	leaves	N6673	CT1811.cel
CT181-1	Treatment: Four 5 ul droplets of sterile potato dextrose broth were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 18 hpi.
CT181-2	2716	leaves	N6673	CT1812.cel
CT181-2	Treatment: Four 5 ul droplets of sterile potato dextrose broth were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 18 hpi.
CT182-1	2717	leaves	N6673	CT1821.cel
CT182-1	Treatment: Four 5 ul droplets of sterile potato dextrose broth were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 18 hpi.
CT481-1	2718	leaves	N6673	CT4811.cel
CT481-1	Treatment: Four 5 ul droplets of sterile potato dextrose broth were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 48 hpi.
CT482-1	2719	leaves	N6673	CT4821.cel
CT482-1	Treatment: Four 5 ul droplets of sterile potato dextrose broth were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 48 hpi.
CT482-2	2720	leaves	N6673	CT4822.cel
CT482-2	Treatment: Four 5 ul droplets of sterile potato dextrose broth were placed on each of 4-5 fully expanded rosette leaves per plant. Plants were watered right before the experiment, leaving 500 ml of watered on the bottom of the flat, then inoculated, covered with a clear plastic lid. Leaves were harvested 48 hpi.