NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:166
Title:Peroxisomal mdh mutant
Date:2005-07-20
Description:The aim is to study the function of peroxisomal NAD-malate dehydrogenase in fatty acid beta-oxidation, glyoxylate cycle and photorespiration. Both peroxisomal MDH genes (At2g22780 and At5g09660) have been knocked out with T-DNA insertions and a double mutant made. Double mutant seedlings are blocked in beta-oxidation - they are 2,4DB resistant and beta-oxidation genes are repressed. They carry out glyoxylate cycle as normal. Plants grow well in soil and produce seed. Microarray analysis will tell us the extent of changes in gene expression in the mutant. For microarray analysis seeds are stratified at 4 C on agar medium with 1/2 strength M&S salts and 1% sucrose for 2 days, then seedlings grown for 2 days at 20 C in the light (100 umol/m2/s). Triplicate samples will be grown for mutant and wild type (col-0) and RNA isolated from each.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Pracharoenwattana_1-1_mdhA_Rep1_ATH12703whole seeds Pracharoenwattana_1-1_mdhA_Rep1_ATH1.CEL
Pracharoenwattana_1-2_mdhB_Rep2_ATH12704whole seeds Pracharoenwattana_1-2_mdhB_Rep2_ATH1.CEL
Pracharoenwattana_1-3_mdhC_Rep3_ATH12705whole seeds Pracharoenwattana_1-3_mdhC_Rep3_ATH1.CEL
Pracharoenwattana_1-4_ColA_Rep1_ATH12706whole seeds Pracharoenwattana_1-4_ColA_Rep1_ATH1.CEL
Pracharoenwattana_1-5_ColB_Rep2_ATH12707whole seeds Pracharoenwattana_1-5_ColB_Rep2_ATH1.CEL
Pracharoenwattana_1-6_ColC_Rep3_ATH12708whole seeds Pracharoenwattana_1-6_ColC_Rep3_ATH1.CEL