NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:163
Title:UV-B response of Arabidopsis
Date:2004-08-23
Description:The UV-B portion of the solar radiation acts as an environmental signal for which plants have evolved specific and sensitive UV-B perception systems. The UV-B-specific UV RESPONSE LOCUS 8 (UVR8) and the multifunctional E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) are key regulators of the UV-B response. We show here that uvr8 null mutants are deficient in UV-B-induced photomorphogenesis and hypersensitive to UV-B stress, whereas overexpression of UVR8 results in enhanced UV-B photomorphogenesis, acclimation and tolerance to UV-B stress. By using sun simulators, we provide evidence at the physiological level that UV-B acclimation mediated by the UV-B-specific photoregulatory pathway is indeed required for survival in sunlight. At the molecular level we demonstrate that the wild type but not the mutant UVR8 and COP1 proteins directly interact in a UV-B-dependent, rapid manner in planta. These data collectively suggest that UV-B-specific interaction of COP1 and UVR8 in the nucleus is a very early step in signalling and responsible for the plants coordinated response to UV-B ensuring UV-B acclimation and protection in the natural environment.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Ulm_1-10_4days-345nm,6hr-305nm_Rep1_ATH12657whole seedling N1092Ulm_1-10_4days-345nm,6hr-305nm_Rep1_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B). This 345 nm cut-off was exchanged with a 305 nm cut-off at 6h before harvesting (i.e. 6h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-11_4days-345nm,6hr-305nm_Rep2_ATH12658whole seedling N1092Ulm_1-11_4days-345nm,6hr-305nm_Rep2_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B). This 345 nm cut-off was exchanged with a 305 nm cut-off at 6h before harvesting (i.e. 6h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-12_4days-345nm,6hr-305nm_Rep3_ATH12659whole seedling N1092Ulm_1-12_4days-345nm,6hr-305nm_Rep3_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B). This 345 nm cut-off was exchanged with a 305 nm cut-off at 6h before harvesting (i.e. 6h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-1_4days-345nm_Rep1_ATH12648whole seedling N1092Ulm_1-1_4days-345nm_Rep1_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W-30 and Philips TL20W-01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B control); UV-B fluence rate: 0 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-2_4days-345nm_Rep2_ATH12649whole seedling N1092Ulm_1-2_4days-345nm_Rep2_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B control); UV-B fluence rate: 0 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-3_4days-345nm_Rep3_ATH12650whole seedling N1092Ulm_1-3_4days-345nm_Rep3_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B control); UV-B fluence rate: 0 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-4_4days-305nm_Rep1_ATH12651whole seedling N1092Ulm_1-4_4days-305m,_Rep1_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 305 nm (i.e. +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-5_4days-305nm_Rep2_ATH12652whole seedling N1092Ulm_1-5_4days-305nm_Rep2_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 305 nm (i.e. +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-6_4days-305nm_Rep3_ATH12653whole seedling N1092Ulm_1-6_4days-305nm_Rep3_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 305 nm (i.e. +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-7_4days-345nm,1hr-305nm_Rep1_ATH12654whole seedling N1092Ulm_1-7_305nm-1h_Rep1_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B). This 345 nm cut-off was exchanged with a 305 nm cut-off at 1h before harvesting (i.e. 1h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-8_4days-345nm,1hr-305nm_Rep2_ATH12655whole seedling N1092Ulm_1-8_4days-345nm,1hr305nm_Rep2_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B). This 345 nm cut-off was exchanged with a 305 nm cut-off at 1h before harvesting (i.e. 1h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_1-9_4days-345nm,1hr-305nm_Rep3_ATH12656whole seedling N1092Ulm_1-9_4days-345nm,1hr-305nm_Rep3_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B). This 345 nm cut-off was exchanged with a 305 nm cut-off at 1h before harvesting (i.e. 1h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_2-10_uvr8-305-4d_Rep1_ATH121300whole seedling Ulm_2-10_uvr8-305-4d_Rep1_ATH1.CEL
Ulm_2-11_uvr8-305-4d_Rep2_ATH121301whole seedling Ulm_2-11_uvr8-305-4d_Rep2_ATH1.CEL
Ulm_2-12_uvr8-305-4d_Rep3_ATH121302whole seedling Ulm_2-12_uvr8-305-4d_Rep3_ATH1.CEL
Ulm_2-1_uvr8-345-4d_Rep1_ATH121291whole seedling N533468Ulm_2-1_uvr8-345-4d_Rep1_ATH1.CEL
Ulm_2-2_uvr8-345-4d_Rep2_ATH121292whole seedling N533468Ulm_2-2_uvr8-345-4d_Rep2_ATH1.CEL
Ulm_2-3_uvr8-345-4d_Rep3_ATH121293whole seedling N533468Ulm_2-3_uvr8-345-4d_Rep3_ATH1.CEL
Ulm_2-4_uvr8-305-1h_Rep1_ATH121294whole seedling N533468Ulm_2-4_uvr8-305-1h_Rep1_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B). This 345 nm cut-off was exchanged with a 305 nm cut-off at 1h before harvesting (i.e. 1h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_2-5_uvr8-305-1h_Rep2_ATH121295whole seedling N533468Ulm_2-5_uvr8-305-1h_Rep2_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B). This 345 nm cut-off was exchanged with a 305 nm cut-off at 1h before harvesting (i.e. 1h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_2-6_uvr8-305-1h_Rep3_ATH121296whole seedling N533468Ulm_2-6_uvr8-305-1h_Rep3_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B). This 345 nm cut-off was exchanged with a 305 nm cut-off at 1h before harvesting (i.e. 1h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_2-7_uvr8-305-6h_Rep1_ATH121297whole seedling N533468Ulm_2-7_uvr8-305-6h_Rep1_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B minus). This 345 nm cut-off was exchanged with a 305 nm cut-off at 6h before harvesting (i.e. 6h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_2-8_uvr8-305-6h_Rep2_ATH121298whole seedling N533468Ulm_2-8_uvr8-305-6h_Rep2_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B minus). This 345 nm cut-off was exchanged with a 305 nm cut-off at 6h before harvesting (i.e. 6h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_2-9_uvr8-305-6h_Rep3_ATH121299whole seedling N533468Ulm_2-9_uvr8-305-6h_Rep3_ATH1.CEL
Treatment: Wild-type Col-0 plants were grown for 4d in a UV-B-supplemented light field (Osram L18W/30 and Philips TL20W/01RS) under a 3-mm cut-off filter of the WG series (Schott, Mainz, Germany) with half-maximal transmission at 345 nm (i.e. UV-B minus). This 345 nm cut-off was exchanged with a 305 nm cut-off at 6h before harvesting (i.e. 6h +UV-B); UV-B fluence rate: 1.5 micromole m-2 s-1, photosynthetically active radiation: 3.3 micromole m-2 s-1.
Ulm_3-10_cop1-305-4d_Rep1_ATH121521whole seedling Ulm_3-10_cop1-305-4d_Rep1_ATH1.CEL
Ulm_3-11_cop1-305-4d_Rep2_ATH121522whole seedling Ulm_3-11_cop1-305-4d_Rep2_ATH1.CEL
Ulm_3-12_cop1-305-4d_Rep3_ATH121523whole seedling Ulm_3-12_cop1-305-4d_Rep3_ATH1.CEL
Ulm_3-1_cop1-345-4d_Rep1_ATH121512whole seedling Ulm_3-1_cop1-345-4d_Rep1_ATH1.CEL
Ulm_3-2_cop1-345-4d_Rep2_ATH121513whole seedling Ulm_3-2_cop1-345-4d_Rep2_ATH1.CEL
Ulm_3-3_cop1-345-4d_Rep3_ATH121514whole seedling Ulm_3-3_cop1-345-4d_Rep3_ATH1.CEL
Ulm_3-4_cop1-305-1h_Rep1_ATH121515whole seedling Ulm_3-4_cop1-305-1h_Rep1_ATH1.CEL
Ulm_3-5_cop1-305-1h_Rep2_ATH121516whole seedling Ulm_3-5_cop1-305-1h_Rep2_ATH1.CEL
Ulm_3-6_cop1-305-1h_Rep3_ATH121517whole seedling Ulm_3-6_cop1-305-1h_Rep3_ATH1.CEL
Ulm_3-7_cop1-305-6h_Rep1_ATH121518whole seedling Ulm_3-7_cop1-305-6h_Rep1_ATH1.CEL
Ulm_3-8_cop1-305-6h_Rep2_ATH121519whole seedling Ulm_3-8_cop1-305-6h_Rep2_ATH1.CEL
Ulm_3-9_cop1-305-6h_Rep3_ATH121520whole seedling Ulm_3-9_cop1-305-6h_Rep3_ATH1.CEL